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首页> 外文期刊>Infection and immunity >Binding Component of Clostridium perfringens Iota-Toxin Induces Endocytosis in Vero Cells
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Binding Component of Clostridium perfringens Iota-Toxin Induces Endocytosis in Vero Cells

机译：产气荚膜梭状芽胞杆菌毒素毒素的结合成分诱导Vero细胞内吞

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摘要

Clostridium perfringens iota-toxin is a binary toxin consisting of two individual proteins, the binding component (Ib) and the enzyme component (Ia). Wild-type Ib bound to Vero cells at 4 and 37°C and formed oligomers at 37°C but not at 4°C. The Ib-induced K+ release from the cells was dependent on the oligomer formation of Ib in the cells, but the oligomer formation did not induce rounding activity or cytotoxicity. After incubation of the cells with recombinant Ib (rIb) at 37°C, the Ib oligomer in the cell became resistant to pronase treatment with time, but the Ib monomer was sensitive to the treatment. Furthermore, treatment of Vero cells with rIb in the presence of bafilomycin, methylamine, or ethylamine resulted in accumulation of the oligomer in the cells but had no effect on K+ release. Moreover, incubation with Ib plus Ia in the presence of these agents caused no rounding in the cells. These observations suggest that Ib binds to Vero cells, itself oligomerizing to form ion-permeable channels and that the formation of oligomer then induces endocytosis.
机译：产气荚膜梭菌（Clostridium perfringens）毒素是一种二元毒素，由两个单独的蛋白质组成，即结合成分（Ib）和酶成分（Ia）。野生型Ib在4和37°C下与Vero细胞结合，并在37°C而非4°C形成寡聚物。由Ib诱导的K + 从细胞中的释放取决于细胞中Ib的寡聚体形成，但寡聚体的形成不会诱导舍入活性或细胞毒性。将细胞与重组Ib（rIb）在37°C孵育后，细胞中的Ib低聚物会随着时间的流逝对链霉蛋白酶的处理产生抗性，但Ib单体对这种处理敏感。此外，在巴氟霉素，甲胺或乙胺的存在下用rIb处理Vero细胞会导致寡聚物在细胞中积累，但对K + 的释放没有影响。而且，在这些试剂的存在下与Ib加Ia一起温育不会引起细胞四舍五入。这些观察结果表明，Ib与Vero细胞结合，其自身寡聚形成离子可渗透的通道，并且寡聚体的形成随后诱导了胞吞作用。

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