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Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells

机译:支气管博德特氏菌中功能性BvgAS毒力控制系统是诱导纤毛气管上皮细胞中Ca2 +瞬变的必要条件

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To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.
机译:为了研究最初的支气管败血博德特氏菌-气管上皮细胞相互作用,我们共孵育了 B。支气管败血病与兔气管外植体培养并检测细菌粘附和宿主细胞Ca 2 + 信号传导。野生型 B。在共孵育的2分钟内,支气管败血病菌(RB50)优先粘附在纤毛上并诱导纤毛宿主细胞Ca 2 + 瞬变,而与无毒力的菌株(RB57)的共孵育导致结合力有限且Ca < sup> 2 + 信令。所描述的小区系统允许评估初始B。支气管败血病菌与宿主细胞的相互作用可能会导致致病性或宿主细胞防御。

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