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首页> 外文期刊>Infection and immunity >Sendai virus glycoproteins are T cell-dependent B cell mitogens.
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Sendai virus glycoproteins are T cell-dependent B cell mitogens.

机译：仙台病毒糖蛋白是依赖T细胞的B细胞有丝分裂原。

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UV-inactivated Sendai virus is mitogenic for murine splenocytes, whereas infectious Sendai virus kills spleen cells in vitro. The isolated hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus are also mitogenic for cultured mouse spleen cells. A mixture of these glycoproteins (1 microgram/well) gives maximum stimulation 96 h after culture initiation. Viral proteins remaining insoluble after Triton X-100 extraction are also mitogenic for mouse spleen cells, with maximum stimulation occurring at 72 h after culture initiation with 1 to 5 microgram/well. On the basis of protein concentration, the HN and F glycoproteins are approximately three times more mitogenic than the Triton X-100-insoluble material. The mitogenic response of the HN and F glycoproteins has two components, a T cell-independent B cell proliferation, which is less than one-half of the total stimulation observed, and a T cell-dependent B cell proliferation. In contrast, the Triton X-100-insoluble material is a T cell-dependent B cell mitogen. Purified T lymphocytes do not respond to the mitogenic signal of either HN-F or Triton X-100-insoluble material.
机译：紫外线灭活的仙台病毒对鼠脾细胞有丝分裂作用，而传染性仙台病毒可在体外杀死脾细胞。仙台病毒的分离的血凝素神经氨酸酶（HN）和融合（F）糖蛋白对培养的小鼠脾细胞也具有促有丝分裂作用。这些糖蛋白的混合物（1微克/孔）在培养开始后96小时给予最大刺激。 Triton X-100提取后保持不溶的病毒蛋白也对小鼠脾细胞有丝分裂作用，最大刺激发生在培养开始后的72小时（1-5微克/孔）。根据蛋白质浓度，HN和F糖蛋白的促有丝分裂作用是不溶于Triton X-100的材料的三倍。 HN和F糖蛋白的促有丝分裂反应具有两个组成部分，即独立于T细胞的B细胞增殖（其不到观察到的总刺激的一半）和依赖于T细胞的B细胞增殖。相反，Triton X-100不溶物是依赖T细胞的B细胞有丝分裂原。纯化的T淋巴细胞不响应HN-F或Triton X-100不溶性物质的促有丝分裂信号。

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