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首页> 外文期刊>Infection and immunity >Further purification and characterization of high-molecular-weight polysaccharide from Pseudomonas aeruginosa.
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Further purification and characterization of high-molecular-weight polysaccharide from Pseudomonas aeruginosa.

机译：铜绿假单胞菌高分子量多糖的进一步纯化和表征。

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Previously published reports on high-molecular-weight polysaccharides from immunotype 1 and 2 of Pseudomonas aeruginosa indicated the presence of high levels of mannose in these preparations. This mannose has been found to be due to the presence of a yeastlike mannan in high-molecular-weight polysaccharide preparations. The source of the mannan was found to be the tryptic soy broth used to grow the bacteria. Mannan could be removed from the polysaccharide preparations by chromatography over columns of concanavalin A-Sepharose. The resulting polysaccharides had the same serological reactivity against rabbit antisera and the same immunogenic properties in mice as did the mannan-containing polysaccharides. Comparison of mannan-depleted polysaccharide with preparations of high-molecular-weight polysaccharide obtained from either ultrafiltered tryptic soy broth or a chemically defined medium showed that these polysaccharides were immunologically and chemically similar. Human immune responses to mannan-depleted polysaccharide from the immunotype 1 strain of P. aeruginosa were comparable with those previously seen in humans receiving mannan-containing polysaccharides. Thus, we found that P. aeruginosa high-molecular-weight polysaccharides prepared in either tryptic soy broth and then subjected to concanavalin A-Sepharose chromatography, ultrafiltered tryptic soy broth, or a chemically defined medium were immunologically and chemically comparable.
机译：先前发表的有关铜绿假单胞菌免疫类型1和2的高分子量多糖的报道表明，这些制剂中存在高水平的甘露糖。已经发现该甘露糖是由于在高分子量多糖制剂中存在酵母样甘露聚糖。发现甘露聚糖的来源是用于生长细菌的胰蛋白酶大豆肉汤。通过在伴刀豆球蛋白A-Sepharose柱上进行色谱法可以从多糖制品中除去甘露聚糖。所得的多糖与含甘露聚糖的多糖具有相同的抗兔抗血清的血清反应性和对小鼠的免疫原性。贫甘露聚糖多糖与从超滤胰蛋白p肉汤或化学成分确定的培养基中获得的高分子量多糖制剂的比较表明，这些多糖在免疫学和化学上均相似。人类对来自铜绿假单胞菌免疫型1株的甘露聚糖枯竭多糖的免疫反应与先前在接受含甘露聚糖多糖的人类中的免疫反应相当。因此，我们发现在胰蛋白酶大豆肉汤中制备的铜绿假单胞菌高分子量多糖，然后进行伴刀豆球蛋白A-琼脂糖层析，超滤胰蛋白酶大豆肉汤或化学成分确定的培养基在免疫学和化学上均具有可比性。

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