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首页> 外文期刊>Infection and immunity >Correlation of the virulence of Klebsiella pneumoniae K1 and K2 with the presence of a plasmid encoding aerobactin.
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Correlation of the virulence of Klebsiella pneumoniae K1 and K2 with the presence of a plasmid encoding aerobactin.

机译：肺炎克雷伯菌K1和K2毒力与编码气杆菌素的质粒的存在相关性。

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Nine isolates of Klebsiella pneumoniae belonging to capsular serotypes K1 and K2 were assayed for virulence in mice. Virulent isolates (50% lethal dose of less than 10(3) microorganisms) and avirulent isolates (50% lethal dose of over 10(6) microorganisms) were selected. Supplementation of a defined minimal medium with transferrin markedly reduced the growth of avirulent strains but had no significant effect on the growth of virulent strains. All isolates produced enterochelin, but only production of aerobactin could be correlated with virulence. The genes encoding aerobactin and its receptor protein were located on a 180-kilobase plasmid. They were cloned into the mobilizable vector pSUP202. Homology was demonstrated with the aerobactin operon of the Escherichia coli plasmid pColV-K30. Transfer of the recombinant plasmid pKP4 into an avirulent recipient enhanced virulence by 100-fold. These experiments demonstrated that aerobactin is an essential factor of pathogenicity in K. pneumoniae.
机译：测定了九种分离物，分别属于荚膜血清型K1和K2的肺炎克雷伯氏菌的毒性。选择强毒分离株（少于10（3）种微生物的50％致死剂量）和无毒分离株（超过10（6）种微生物的50％致死剂量）。用转铁蛋白补充确定的基本培养基显着降低了无毒力菌株的生长，但对有毒力菌株的生长没有显着影响。所有分离株均产生肠螯合素，但只有气杆菌素的产生可能与毒力有关。编码航空杆菌素及其受体蛋白的基因位于180碱基对的质粒上。将它们克隆到可移动载体pSUP202中。大肠杆菌质粒pColV-K30的航空杆菌素操纵子证实了同源性。将重组质粒pKP4转移到无毒受体中可将毒力提高100倍。这些实验证明，航空细菌素是肺炎克雷伯菌中致病性的重要因素。

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