Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli.

S L Morris; D A Rouse; D Hussong; S D Chaparas

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Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli.

机译：重组λgt11噬菌体的分离和鉴定，该噬菌体在大肠杆菌中表达免疫反应性胞内分枝杆菌蛋白。

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Disseminated Mycobacterium avium-Mycobacterium intracellulare (M. avium complex) disease is a prevalent opportunistic infection in patients with acquired immune deficiency syndrome. Because of the increasing importance of this disease, an M. avium complex lambda gt11 expression library was prepared. We screened the library with an absorbed anti-M. intracellulare serum and identified a recombinant phage which expressed a 190-kilodalton beta-galactosidase-M. intracellulare fusion protein. Lysates containing the 190-kilodalton fusion protein evoked strong humoral and cell-mediated responses. The immunoreactivity of the M. intracellulare recombinant protein suggests that antigens isolated from the expression library may be useful as skin test, serodiagnostic, or immunoprophylactic reagents for M. avium complex disease.

机译：传播性鸟分枝杆菌-细胞内分枝杆菌（鸟分枝杆菌复合体）疾病是获得性免疫缺陷综合征患者的一种普遍的机会感染。由于该疾病的重要性日益增加，因此制备了鸟分枝杆菌复杂λgt11表达文库。我们用吸收的抗M筛选了文库。细胞内血清并鉴定出表达190-千达尔顿β-半乳糖苷酶-M的重组噬菌体。细胞内融合蛋白。含有190-千达尔顿融合蛋白的裂解物引起强烈的体液和细胞介导的反应。细胞内分枝杆菌重组蛋白的免疫反应性表明，从表达文库中分离的抗原可用作禽分枝杆菌复杂疾病的皮肤测试，血清学诊断或免疫预防试剂。

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《Infection and immunity》 |1988年第12期|共6页
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S L Morris; D A Rouse; D Hussong; S D Chaparas;
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中图分类医学免疫学;
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1. Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli. [J] . S L Morris, D A Rouse, D Hussong, Infection and immunity . 1988,第12期

机译：重组λgt11噬菌体的分离和鉴定，该噬菌体在大肠杆菌中表达免疫反应性胞内分枝杆菌蛋白。
2. Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens. [J] . S L Morris, D A Rouse, D Hussong, Infection and immunity . 1990,第1期

机译：表达四种不同细胞内分枝杆菌抗原的重组λgt11噬菌体的分离和鉴定。
3. Isolation and characterization of recombinant lambda gt11 bacteriophages expressing eight different mycobacterial antigens of potential immunological relevance. [J] . A B Andersen, A Worsaae, S D Chaparas Infection and immunity . 1988,第5期

机译：表达八种潜在免疫学相关分枝杆菌抗原的重组λgt11噬菌体的分离和鉴定。
4. Expression of Functional Recombinant Barnacle Cement Protein Bacp-20k in Escherichia coli. [C] . Sun Yanan, Du Lina, Jiang Zhen, International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：函数重组晶粒水泥蛋白Bacp-20k在大肠杆菌中的表达。
5. Improving Co-Translational Incorporation of Non-Standard Amino Acids into Recombinant Proteins in Escherichia coli. [D] . Perez, Jessica Gabrielle. 2017

机译：改善大肠杆菌中非标准氨基酸到重组蛋白的共翻译结合。
6. Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli. [O] . S L Morris, D A Rouse, D Hussong, 1988

机译：重组λgt11噬菌体的分离和鉴定该噬菌体在大肠杆菌中表达免疫反应性胞内分枝杆菌蛋白。
7. Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli [O] . S L Morris, D A Rouse, D Hussong, 1988

机译：重组λGT11噬菌体的分离和表征，其表达大肠杆菌中的免疫反应性分枝杆菌蛋白质
8. Identification and Characterization of Mutations in 'Escherichia coli' That Selectively Influence the Growth of Hybrid Lambda Bacteriophages Carrying the Immunity Region of Bacteriophage P22 [R] . Strauch, M. A., Baumann, M., Friedman, D. I., 1986

机译：“大肠杆菌”突变的鉴定和表征，选择性地影响携带噬菌体p22免疫区的杂种λ噬菌体的生长

Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli.

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