Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens.

S L Morris; D A Rouse; D Hussong; S D Chaparas

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Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens.

机译：表达四种不同细胞内分枝杆菌抗原的重组λgt11噬菌体的分离和鉴定。

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Four bacteriophages expressing different immunoreactive recombinant Mycobacterium intracellulare antigens were isolated from a lambda gt11 library with monoclonal antibodies to M. intracellulare. These four antibodies reacted with native M. intracellulare proteins of 54, 43, 40/38, and 22 kilodaltons. Southern blot hybridizations with DNA probes prepared from insert fragments of these bacteriophages confirmed the M. intracellulare derivation of the inserts. The physical maps of the immunoreactive phages were deduced by restriction enzyme digestions. The molecular weights of the expressed recombinant antigens were determined by Western (immuno-) blotting.

机译：用抗胞内分枝杆菌的单克隆抗体从λgt11文库中分离出四种表达不同免疫反应性重组胞内分枝杆菌抗原的噬菌体。这四种抗体与54、43、40 / 38和22千道尔顿的天然胞内分枝杆菌蛋白反应。用由这些噬菌体的插入片段制备的DNA探针进行的Southern印迹杂交证实了插入片段的胞内分枝杆菌。免疫反应噬菌体的物理图谱通过限制性内切酶消化得到。通过Western（免疫）印迹法测定表达的重组抗原的分子量。

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《Infection and immunity》 |1990年第1期|共4页
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S L Morris; D A Rouse; D Hussong; S D Chaparas;
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中图分类医学免疫学;
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1. Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli. [J] . S L Morris, D A Rouse, D Hussong, Infection and immunity . 1988,第12期

机译：重组λgt11噬菌体的分离和鉴定，该噬菌体在大肠杆菌中表达免疫反应性胞内分枝杆菌蛋白。
2. Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli. [J] . S L Morris, D A Rouse, D Hussong, Infection and immunity . 1988,第12期

机译：重组λgt11噬菌体的分离和鉴定，该噬菌体在大肠杆菌中表达免疫反应性胞内分枝杆菌蛋白。
3. Isolation and characterization of recombinant lambda gt11 bacteriophages expressing eight different mycobacterial antigens of potential immunological relevance. [J] . A B Andersen, A Worsaae, S D Chaparas Infection and immunity . 1988,第5期

机译：表达八种潜在免疫学相关分枝杆菌抗原的重组λgt11噬菌体的分离和鉴定。
4. Optimization of Recombinant Bacteriophage Lysin of Streptococcus Expression in Escherichia coli [C] . Wang Pu, Wang Xiaofeng, Chen Weiqing 2010 4th International Conference on Bioinformatics and Biomedical Engineering . 2010

机译：重组链球菌噬菌体溶菌素在大肠杆菌中表达的优化
5. Expression, purification and characterization of bacteriophage lambda tail tip proteins. [D] . Dai, Xiaoxian. 2009

机译：噬菌体λ尾尖蛋白的表达，纯化和鉴定。
6. Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens. [O] . S L Morris, D A Rouse, D Hussong, 1990

机译：表达四种不同胞内分枝杆菌抗原的重组λgt11噬菌体的分离和鉴定。
7. Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli [O] . S L Morris, D A Rouse, D Hussong, 1988

机译：重组λGT11噬菌体的分离和表征，其表达大肠杆菌中的免疫反应性分枝杆菌蛋白质

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Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens.

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