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首页> 外文期刊>Infection and immunity >Lethal infection by Bordetella pertussis mutants in the infant mouse model.
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Lethal infection by Bordetella pertussis mutants in the infant mouse model.

机译：百日咳博德特氏菌突变体在婴儿小鼠模型中致死性感染。

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Different aspects of lethal infection of infant mice with Bordetella pertussis were examined. Mutants deficient in vir-regulated genes were tested for the ability to cause a lethal infection in the infant mouse model. Adenylate cyclase toxin-hemolysin and pertussis toxin were required to cause a lethal infection at low doses. Mixed infection caused by challenging the mice with an equal number of pertussis toxin and adenylate cyclase toxin-hemolysin mutants at a dose at which neither alone was lethal was also unable to cause a lethal infection. Production of the filamentous hemagglutinin and the dermonecrotic toxin was not required to cause a lethal infection. Nine other mutants in vir-regulated genes whose phenotypes have yet to be determined were also tested. Only two of these mutants were impaired in the ability to cause a lethal infection. Expression of fimbriae does not appear to affect the dose required to cause a lethal infection; however, fimbrial expression was correlated with the later stages of a nonlethal, persistent infection. Growth of the bacteria in MgSO4, a condition which reversibly suppresses expression of the genes required for virulence, did not alter the ability of the bacteria to cause a lethal infection. Auxotrophic mutants deficient in leucine biosynthesis were as virulent as the parental strain; however, mutants deficient in methionine biosynthesis were less virulent. A B. parapertussis strain was much less effective in promoting a lethal infection than any of the wild-type B. pertussis strains examined. A persistent infection in the lungs was observed for weeks after challenge for mice given a sublethal dose of B. pertussis, and transmission from infected infants to the mother was never observed.
机译：检查了百日咳博德特氏菌对婴儿小鼠致死性感染的不同方面。测试了缺乏病毒调节基因的突变体在婴儿小鼠模型中引起致命感染的能力。需要使用腺苷酸环化酶毒素-溶血素和百日咳毒素以低剂量引起致命感染。用相同数量的百日咳毒素和腺苷酸环化酶毒素-溶血素突变体攻击小鼠而引起的混合感染，既不能单独致死，也不能引起致死性感染。不需要产生丝状血凝素和皮肤坏死毒素来引起致命的感染。还测试了其表型尚未确定的病毒调节基因中的其他九个突变体。这些突变体中只有两个突变体导致致命感染的能力受损。菌毛的表达似乎并不影响引起致命感染所需的剂量。然而，纤维表达与非致死性持续感染的后期有关。细菌在MgSO4中的生长可逆地抑制了毒力所需基因的表达，但这种条件并没有改变细菌引起致命感染的能力。缺乏亮氨酸生物合成的营养缺陷型突变体与亲本菌株一样有毒力。但是，缺乏蛋氨酸生物合成的突变体毒性较低。与所检查的任何野生型百日咳博德特氏菌菌株相比，副百日咳博德特氏菌菌株在致死性感染方面的效力要低得多。在给予亚致死剂量百日咳博德特氏菌的小鼠攻击后数周，观察到肺部持续感染，从未观察到从受感染的婴儿向母亲的传播。

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