• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Identification of hemolytic and cytotoxic proteins of Actinobacillus pleuropneumoniae by use of monoclonal antibodies.
【24h】

Identification of hemolytic and cytotoxic proteins of Actinobacillus pleuropneumoniae by use of monoclonal antibodies.

机译：通过使用单克隆抗体鉴定胸膜肺炎放线杆菌的溶血和细胞毒性蛋白。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Monoclonal antibodies (MAbs) were raised against extracellular hemolytic and cytotoxic proteins of Actinobacillus pleuropneumoniae serotypes 2 and 9. MAbs were tested for inhibition of hemolytic and cytotoxic activities of the reference strains of A. pleuropneumoniae serotypes 1 to 12. Five MAbs inhibited hemolytic or cytotoxic activities of one or more serotypes and were used to probe Western blots (immunoblots) of cytotoxic and hemolytic culture filtrates of serotypes 1 to 12. The MAbs recognized three different proteins: (i) a protein of approximately 103 kDa that was associated with a weak hemolytic activity and a moderate cytotoxic activity, (ii) a protein of approximately 105 kDa that was associated with a strong hemolytic activity and a strong cytotoxic activity, and (iii) a protein of approximately 120 kDa that was associated with a strong cytotoxic activity but not with hemolytic activity. Serotypes 6, 7, and 12 produced only the 103-kDa protein, and serotype 10 produced only the 105-kDa protein. The other serotypes produced two proteins: serotypes 1, 5, 9, and 11 produced the 103- and 105-kDa proteins, and serotypes 2, 3, 4, and 8 produced the 103- and 120-kDa proteins.
机译：产生了针对胸膜肺炎放线杆菌血清型2和9的细胞外溶血和细胞毒性蛋白的单克隆抗体（MAb）。测试了单克隆抗体对胸膜肺炎放线杆菌血清型1至12的参考菌株的溶血和细胞毒活性的抑制作用。五种MAb抑制了溶血或细胞毒作用具有一种或多种血清型的活性，并用于探测1至12型血清毒性和溶血培养滤液的Western印迹（免疫印迹）.MAb识别三种不同的蛋白：（​​i）约103 kDa的蛋白与弱蛋白相关溶血活性和中等的细胞毒性活性;（ii）大约105 kDa的蛋白具有很强的溶血活性和强烈的细胞毒性活性，以及​​（iii）大约120 kDa的蛋白具有很强的细胞毒性活性，但是不具有溶血活性。血清型6、7和12仅产生103 kDa蛋白，血清型10仅产生105 kDa蛋白。其他血清型产生两种蛋白质：血清型1、5、9和11产生103和105 kDa的蛋白质，血清型2、3、4和8产生103和120 kDa的蛋白质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号