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首页> 外文期刊>Infection and immunity >Tumor necrosis factor and macrophage activation are important in clearance of Nocardia brasiliensis from the livers and spleens of mice.
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Tumor necrosis factor and macrophage activation are important in clearance of Nocardia brasiliensis from the livers and spleens of mice.

机译：肿瘤坏死因子和巨噬细胞激活对于从小鼠的肝脏和脾脏清除巴西诺卡氏菌很重要。

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The roles of tumor necrosis factor (TNF) and macrophage activation in clearance of Nocardia brasiliensis from BALB/c mouse livers and spleens were evaluated. TNF activity was detectable in sera from animals at all stages of infection. Treatment of infected mice with an antiserum against TNF significantly enhanced the experimental infection as judged by enumeration of CFU in the spleens and livers of infected mice. In another set of experiments, a population of activated macrophages from the peritoneal cavities of N. brasiliensis-infected mice was studied by using a cytostatic assay. The observed cytotoxic activity of these activated macrophages against L929 cells was mediated by TNF, since this activity was inhibited by anti-TNF antiserum treatment. The level of TNF activity generated in vitro in the presence of lipopolysaccharide (LPS) by peritoneal macrophages from infected mice was higher than that of adherent peritoneal cells obtained from normal mice after challenge with LPS. When the nocardiacidal activity of peritoneal cells from N. brasiliensis-infected mice was estimated in vitro, a significant decrease in the number of CFU recovered was observed. Moreover, nocardiacidal activity of peritoneal cells obtained from N. brasiliensis-infected mice previously treated with anti-TNF antiserum was significantly reduced compared with the activity of cells obtained from infected mice previously treated with normal rabbit serum and that of cells from uninfected mice. These data suggest a role for TNF in resistance to N. brasiliensis infection.
机译：评估了肿瘤坏死因子（TNF）和巨噬细胞激活在从BALB / c小鼠肝脏和脾脏清除巴西诺卡氏菌中的作用。在感染的所有阶段，在动物血清中均可检测到TNF活性。根据对感染小鼠脾脏和肝脏中CFU的计数判断，用抗TNF的抗血清治疗感染小鼠可显着增强实验性感染。在另一组实验中，通过使用细胞抑制试验研究了来自巴西新孢子虫感染小鼠腹膜腔的活化巨噬细胞群。这些活化的巨噬细胞对L929细胞的观察到的细胞毒活性是由TNF介导的，因为这种活性被抗TNF抗血清治疗所抑制。感染小鼠的腹膜巨噬细胞在脂多糖（LPS）存在下体外产生的TNF活性水平高于LPS攻击后从正常小鼠获得的粘附性腹膜细胞水平。当在体外估计来自巴西新孢子虫感染的小鼠的腹膜细胞的抗心酸活性时，观察到回收的CFU数量显着降低。而且，与从先前用正常兔血清处理过的感染小鼠和未感染小鼠的细胞中得到的细胞活性相比，从先前用抗TNF抗血清治疗过的巴西新孢子虫感染的小鼠中得到的腹膜细胞的抗心酸活性显着降低。这些数据表明TNF在对巴西柔毛猪笼草感染的抗性中的作用。

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