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首页> 外文期刊>Infection and immunity >Hydrophobic surface protein masking by the opportunistic fungal pathogen Candida albicans.
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Hydrophobic surface protein masking by the opportunistic fungal pathogen Candida albicans.

机译：疏水性表面蛋白被机会性真菌病原体白色念珠菌掩盖。

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Ultrastructural and biochemical analyses of hydrophobic and hydrophilic yeast cell surface proteins of Candida albicans were performed. Hydrophobic and hydrophilic yeast cells were obtained by growth at 23 and 37 degrees C, respectively. In addition, hydrophilic yeast cells were converted to surface hydrophobicity by treatment with tunicamycin and dithiothreitol. When freeze-etched cells were examined, the temperature-induced hydrophilic cells had long (0.198 micron), compact, evenly distributed fibrils while temperature-induced hydrophobic cells had short (0.085 micron), blunt fibrils. Hydrophobic microsphere attachment to the hydrophobic cells occurred at the basement of and within the short fibril layer. Dithiothreitol-induced hydrophobic cells had the long fibrils removed; tunicamycin-induced hydrophobic cells retained some of the long fibrils, but the fibrils were less compact and more aggregated than the untreated controls. These results suggest that the long fibrils prevent hydrophobic microsphere attachment to the hydrophobic area of the cell surface. This was confirmed by assessing the hydrophobic avidity of hydrophobic yeast cell populations differing in fibril density and arrangement. 125I-labelled surface proteins from hydrophobic and hydrophilic cells were compared after separation by hydrophobic interaction chromatography-high-performance liquid chromatography and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The yeast cell populations had hydrophilic proteins of similar molecular masses (greater than 200 kDa), but the hydrophilic cells possessed at least two additional proteins (ca. 63 and 69 to 71 kDa). Hydrophobic surface proteins appeared to be similar. However, the amount of total radiolabelled hydrophobic proteins was approximately 10-fold higher for the hydrophobic cells than for the hydrophilic cells. This result agrees with the ultrastructural observations which showed that yeast cell surface hydrophobic proteins are masked by hydrophilic high-molecular-mass surface fibrils. Taken together, the data indicate that yeast cell hydrophobicity is not determined by differences in surface hydrophobic proteins but by the presence of hydrophilic, surface fibrils.
机译：对白色念珠菌的疏水和亲水酵母细胞表面蛋白进行了超微结构和生化分析。通过分别在23和37℃下生长获得疏水和亲水酵母细胞。另外，通过用衣霉素和二硫苏糖醇处理将亲水性酵母细胞转化为表面疏水性。当检查冷冻蚀刻的细胞时，温度诱导的亲水性细胞具有长（0.198微米），致密，均匀分布的原纤维，而温度诱导的疏水性细胞具有短（0.085微米），钝的原纤维。疏水性微球与疏水性细胞的附着发生在短纤维层的底部和内部。二硫苏糖醇诱导的疏水性细胞去除了长纤维。衣霉素诱导的疏水性细胞保留了一些长原纤维，但与未处理的对照相比，原纤维较不致密且聚集更多。这些结果表明，长纤维阻止疏水微球附着到细胞表面的疏水区域。通过评估在原纤维密度和排列上不同的疏水酵母细胞群的疏水亲和力可以证实这一点。通过疏水相互作用色谱-高效液相色谱分离后，比较了疏水和亲水细胞中125 I标记的表面蛋白，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影分析。酵母细胞群具有相似分子量（大于200 kDa）的亲水性蛋白，但是亲水性细胞具有至少两种其他蛋白（约63和69至71 kDa）。疏水表面蛋白似乎相似。但是，疏水细胞的总放射性标记疏水蛋白的量比亲水细胞高约10倍。该结果与超微结构观察结果一致，超微结构观察结果表明，酵母细胞表面疏水蛋白被亲水的高分子表面纤维所掩盖。总体而言，数据表明酵母细胞的疏水性不是由表面疏水蛋白的差异决定，而是由亲水性表面原纤维的存在决定。

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