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首页> 外文期刊>Infection and immunity >Legionella pneumophila lipopolysaccharide activates the classical complement pathway.
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Legionella pneumophila lipopolysaccharide activates the classical complement pathway.

机译：嗜肺军团菌脂多糖可激活经典补体途径。

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Legionella pneumophila is a gram-negative bacterium capable of entering and growing in alveolar macrophages and monocytes. Complement and complement receptors are important in the uptake of L. pneumophila by human mononuclear phagocytes. The surface molecules of L. pneumophila that activate the complement system are unknown. To identify these factors, we investigated the effects of L. pneumophila lipopolysaccharide (LPS) on the classical and alternative complement pathways of normal human serum by functional hemolytic assays. Although incubation of LPS in normal human serum at 37 degrees C resulted in the activation of both pathways, complement activation proceeded primarily through the classical pathway. Activation of the classical pathway by LPS was dependent on natural antibodies of the immunoglobulin M class that were present in various quantities in sera from different normal individuals but were absent in an immunoglobulin-deficient serum obtained from an agammaglobulinemic patient. Additional studies using sheep erythrocytes coated with LPS suggested that the antibodies recognized antigenic sites in the carbohydrate portion of LPS. The ability of LPS to interact with the complement system suggests a role for LPS in the uptake of L. pneumophila by mononuclear phagocytes.
机译：嗜肺军团菌是革兰氏阴性细菌，能够进入肺泡巨噬细胞和单核细胞并在其中生长。补体和补体受体在人单核吞噬细胞摄取嗜肺乳杆菌中很重要。激活补体系统的嗜肺乳杆菌的表面分子是未知的。为了确定这些因素，我们通过功能性溶血试验研究了嗜肺乳杆菌脂多糖（LPS）对正常人血清经典途径和替代途径的影响。尽管在37摄氏度的正常人血清中LPS的孵育会导致两种途径的激活，但补体的激活主要通过经典途径进行。 LPS对经典途径的激活取决于免疫球蛋白M类的天然抗体，该抗体以不同量存在于来自不同正常个体的血清中，但在缺乏血球蛋白血症患者的免疫球蛋白缺陷血清中却不存在。使用涂有LPS的绵羊红细胞进行的其他研究表明，抗体识别LPS碳水化合物部分中的抗原位点。 LPS与补体系统相互作用的能力表明LPS在单核吞噬细胞摄取嗜肺乳杆菌中具有作用。

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