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首页> 外文期刊>Infection and immunity >Effects of Listeria monocytogenes and Yersinia enterocolitica on cytokine gene expression and release from human polymorphonuclear granulocytes and epithelial (HEp-2) cells.
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Effects of Listeria monocytogenes and Yersinia enterocolitica on cytokine gene expression and release from human polymorphonuclear granulocytes and epithelial (HEp-2) cells.

机译：单核细胞增生李斯特菌和小肠结肠炎耶尔森氏菌对人多形核粒细胞和上皮（HEp-2）细胞中细胞因子基因表达和释放的影响。

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摘要

The gene expression and cytokine release of the proinflammatory cytokines interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) after infection of human epithelial cells (HEp-2 cells) and polymorphonuclear granulocytes (PMNs) were investigated by using isogenic pairs of Listeria monocytogenes and Yersinia enterocolitica strains. By polymerase chain reaction-assisted mRNA amplification and RNA dot blot analysis, we showed that PMNs and HEp-2 cells expressed enhanced levels of mRNA encoding IL-1 beta, IL-6, and TNF-alpha after bacterial infection. Concomitant with the enhanced mRNA level, an increased secretion rate of IL-1 beta, IL-6, and TNF-alpha from PMNs as assessed by enzyme-linked immunosorbent assay was observed. HEp-2 cells after infection also released IL-6 and TNF-alpha into the cell supernatant, while no IL-1 beta release was detected. Cellular coincubation experiments were carried out with Transwell chambers. Our studies revealed that the coculture of PMNs and HEp-2 cells led to an increased IL-1 beta and IL-6 release. In contrast, after infection with the invasive bacteria, reduced levels of TNF-alpha were measured. Our data show that PMNs secrete the proinflammatory cytokines IL-1 beta, IL-6, and TNF-alpha within some hours after infection with L. monocytogenes and Y. enterocolitica and that cellular interactions with epithelial cells alone via soluble mediators influence the net amount of released proinflammatory cytokines.
机译：感染人类上皮细胞（HEp-2细胞）和多形核粒细胞后促炎细胞因子白介素-1β（IL-1 beta），IL-6和肿瘤坏死因子α（TNF-alpha）的基因表达和细胞因子释放（PMNs）通过使用单核细胞增生李斯特菌和肠球菌耶尔森菌的同基因对进行研究。通过聚合酶链反应辅助的mRNA扩增和RNA点印迹分析，我们发现细菌感染后PMN和HEp-2细胞表达的IL-1β，IL-6和TNF-α的mRNA表达水平提高。通过酶联免疫吸附法评估，与增加的mRNA水平同时，观察到PMN中IL-1β，IL-6和TNF-α的分泌增加。感染后的HEp-2细胞也将IL-6和TNF-α释放到细胞上清液中，而未检测到IL-1β释放。用Transwell小室进行细胞共孵育实验。我们的研究表明，PMN和HEp-2细胞的共培养导致IL-1β和IL-6释放增加。相反，感染性细菌感染后，TNF-α水平降低。我们的数据显示，PMNs在感染单核细胞增生李斯特菌和小肠结肠炎耶尔森氏菌后数小时内分泌促炎细胞因子IL-1β，IL-6和TNF-α，并且仅通过可溶性介质与上皮细胞发生细胞相互作用会影响净含量释放的促炎细胞因子。

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