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首页> 外文期刊>Infection and immunity >Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses.
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Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses.

机译：梅毒螺旋体亚种，毒株和其他致病性色氨酸中15公斤级脂蛋白的保守性：遗传和抗原分析。

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The 15-kDa lipoprotein of Treponema pallidum is a major immunogen during natural syphilis infection in humans and experimental infection in other hosts. The humoral and cellular immune responses to this molecule appear late in infection as resistance to reinfection is developing. One therefore might hypothesize that this antigen is important for protective immunity. This possibility is explored by using both genetic and antigenic approaches. Limited or no cross-protection has been demonstrated between the T. pallidum subspecies and strains or between Treponema species. We therefore hypothesized that if the 15-kDa antigen was of major importance in protective immunity, it might be a likely site of antigenic diversity. To explore this possibility, the sequences of the open reading frames of the 15-kDa gene have been determined for Treponema pallidum subsp. pallidum (Nichols and Bal-3 strains), T. pallidum subsp. pertenue (Gauthier strain), T. pallidum subsp. endemicum (Bosnia strain), Treponema paraluiscuniculi (Cuniculi A, H, and K strains), and a little-characterized simian isolate of Treponema sp. (Fribourg-Blanc strain). No significant differences in DNA sequences of the genes for the coding region of the 15-kDa antigen were found among the different species and subspecies studied. In addition, all organisms showed expression of the 15-kDa antigen as determined by monoclonal antibody staining. The role of the 15-kDa antigen in protection against homologous infection with T. pallidum subsp. pallidum Nichols was examined in rabbits immunized with a purified recombinant 15-kDa fusion protein. No alteration in chancre development was observed in immunized, compared to unimmunized, rabbits, and the antisera induced by the immunization failed to enhance phagocytosis of T. pallidum subsp. pallidum by macrophages in vitro. These results do not support a major role for this antigen in protection against syphilis infection.
机译：梅毒螺旋体的15 kDa脂蛋白是人类天然梅毒感染和其他宿主实验感染期间的主要免疫原。随着对再感染的抵抗力的发展，对该分子的体液和细胞免疫反应在感染后期出现。因此，可以假设该抗原对于保护性免疫很重要。通过使用遗传和抗原方法都探索了这种可能性。在苍白锥虫亚种和菌株之间或梅毒螺旋体物种之间已证明有限或没有交叉保护。因此，我们假设，如果15 kDa抗原在保护性免疫中起着重要作用，则可能是抗原多样性的一个可能位点。为了探索这种可能性，已经确定了梅毒螺旋体亚种的15-kDa基因的开放阅读框的序列。 pallidum（Nichols和Bal-3株），T. pallidum subsp。百日咳（Gauthier株），苍白锥虫亚种。 Endemicum（波斯尼亚毒株），副螺旋体（Cuniculi A，H和K毒株）和一种特征很少的猿猴隔离体。 （弗里堡-布兰克株）。在所研究的不同物种和亚种之间，未发现15-kDa抗原编码区基因的DNA序列的显着差异。另外，通过单克隆抗体染色确定，所有生物均显示15-kDa抗原的表达。 15 kDa抗原在预防苍白锥虫亚种同源感染中的作用。在用纯化的重组15-kDa融合蛋白免疫的兔子中检查了苍白的Nichols。与未免疫的兔子相比，在免疫中未观察到下颚发育的改变，并且由免疫诱导的抗血清不能增强苍白锥虫亚种的吞噬作用。苍白球经巨噬细胞体外培养。这些结果不支持该抗原在预防梅毒感染中的主要作用。

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