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首页> 外文期刊>Infection and immunity >Preparation of a nontoxic and immunogenic polysaccharide fraction from a Haemophilus influenzae phenol-water extract.
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Preparation of a nontoxic and immunogenic polysaccharide fraction from a Haemophilus influenzae phenol-water extract.

机译：从流感嗜血杆菌苯酚-水提取物中制备无毒且具有免疫原性的多糖级分。

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A phenol-water extract from Haemophilus influenzae type a was hydrolyzed to decrease the toxicity without affecting the antigenicity of the preparation. We used partial hydrolysis for 15 h with ion exchangers in the presence of chloroform. The lipid fraction was collected into the organic solvent. The preparation obtained from the aqueous solution was designated the polysaccharide fraction. Rhamnose, glucose, galactose, mannose, and glucosamine were the major components of the polysaccharide fraction, and their molar ratios were determined by gas-liquid chromatography; 2.5% myristic acid was also found in the polysaccharide fraction. The mild hydrolysis of the polysaccharide fraction for 15 h caused a marked reduction in toxicity (50% lethal dose, 183 +/- 9 microgram/kg) and pyrogenicity. The generalized Sanarelli reaction was negative. The local Shwartzman phenomenon was not observed if chloroform and Dowex were exchanged three times during hydrolysis. Most of the antigenic components remained active after the hydrolytic process. The polysaccharide fraction could also induce the formation of circulating antibodies in rabbits and also increase the phagocytic process against H. influenzae from month 2 to 6.
机译：水解甲型流感嗜血杆菌的酚水提取物以降低毒性，而不影响制剂的抗原性。在氯仿存在下，我们与离子交换剂一起使用了15小时的部分水解反应。将脂质级分收集到有机溶剂中。从水溶液获得的制剂称为多糖级分。鼠李糖，葡萄糖，半乳糖，甘露糖和葡糖胺是多糖级分的主要成分，其摩尔比通过气-液色谱法测定。在多糖级分中也发现了2.5％肉豆蔻酸。多糖级分的温和水解15小时导致毒性（50％致死剂量，183 +/- 9微克/ kg）和热原性显着降低。广义的Sanarelli反应为阴性。如果在水解过程中将氯仿和Dowex交换3次，则未观察到局部Shwartzman现象。水解过程后，大多数抗原成分保持活性。多糖级分还可以诱导兔中循环抗体的形成，并且还将针对流感嗜血杆菌的吞噬过程从第2个月增加到第6个月。

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