...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Differences in adhesion of Pseudomonas aeruginosa to mucin glycopeptides from sputa of patients with cystic fibrosis and chronic bronchitis.
【24h】

Differences in adhesion of Pseudomonas aeruginosa to mucin glycopeptides from sputa of patients with cystic fibrosis and chronic bronchitis.

机译：铜绿假单胞菌与囊性纤维化和慢性支气管炎患者痰液粘蛋白糖肽粘附的差异。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pseudomonas aeruginosa is the most prominent colonizer of the respiratory tract of patients with cystic fibrosis, but it is not known why this occurs. P. aeruginosa adheres to mucins from normal individuals, but mucins from cystic fibrosis patients have not been studied. To compare adhesion to mucins from cystic fibrosis with other mucins, we prepared highly glycosylated mucin glycopeptides from cystic fibrosis and chronic bronchitis patients by ion-exchange and gel-filtration chromatography and measured the adhesion of P. aeruginosa 1244 to these glycopeptides. We found (i) that the most mucinlike glycopeptides from P. aeruginosa-infected cystic fibrosis sputa showed less bacterial adhesion than did the corresponding bronchitis samples, (ii) that the most adhesive activity in cystic fibrosis samples came from a fraction that contains O and N glycopeptides and may be in part a degradation product of P. aeruginosa infection, and (iii) that highly glycosylated glycopeptides of the most acidic species (sialylated and sulfated) showed no adhesion at all. A single cystic fibrosis sample not infected by P. aeruginosa showed better binding in the adhesion-positive fractions than did the infected sputa. These studies suggest that cystic fibrosis mucins may be altered after infection is established, resulting in less binding to some fragments. However, since the clinical picture shows heavy mucus colonization, other receptors, such as cellular glycolipids which have been shed into mucus, may be contributing to this colonization.
机译：铜绿假单胞菌是囊性纤维化患者呼吸道最突出的定居者，但尚不知道为什么会发生这种现象。铜绿假单胞菌粘附于正常人的粘蛋白，但尚未研究来自囊性纤维化患者的粘蛋白。为了比较囊性纤维化与其他粘蛋白对黏蛋白的黏附性，我们通过离子交换和凝胶过滤色谱法从囊性纤维化和慢性支气管炎患者中制备了高度糖基化的黏蛋白糖肽，并测量了铜绿假单胞菌1244对这些糖肽的黏附性。我们发现（i）铜绿假单胞菌感染的囊性纤维化痰中黏蛋白样糖肽含量最高，与相应的支气管炎样品相比，细菌黏附性降低;（ii）囊性纤维化样品中黏附性最高的部分来自含有O和N个糖肽，可能部分是铜绿假单胞菌感染的降解产物，以及（iii）最酸性物种（唾液酸化和硫酸化）的高度糖基化糖肽根本没有粘附。未被铜绿假单胞菌感染的单个囊性纤维化样品显示出比感染的痰液更好的结合阳性组分中的结合。这些研究表明，感染建立后，囊性纤维化粘蛋白可能会发生改变，从而导致与某些片段的结合减少。但是，由于临床表现为粘液大量移生，因此其他受体（例如掉入粘液中的细胞糖脂）可能有助于这种移生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号