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首页> 外文期刊>Infection and immunity >Listeria monocytogenes isolates can be classified into two major types according to the sequence of the listeriolysin gene.
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Listeria monocytogenes isolates can be classified into two major types according to the sequence of the listeriolysin gene.

机译：根据李斯特菌溶血素基因的序列，单核细胞增生李斯特菌分离株可分为两种主要类型。

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摘要

The nucleotide sequence of a 3.5-kb BamHI fragment from Listeria monocytogenes 12067, a human clinical isolate of serotype 4b, has been determined. The DNA fragment harbors the gene for listeriolysin, part of the gene for a phosphatidylinositol-specific phospholipase C, and part of the gene for a metalloprotease. Comparison of the sequence with corresponding sequences from two other L. monocytogenes isolates revealed a significant number of nucleotide differences. Several of the differences give rise to amino acid substitutions. The most variable region was the examined part of the mpl gene, whereas the lisA gene showed a relatively high degree of conservation, particularly at the amino acid level. To analyze the pattern of sequence variability in the lisA gene, a 160-bp region covering nine nucleotide differences was sequenced from 36 isolates of different origins. This work showed that the strains can be grouped into two major types according to the nucleotide sequences. Oligonucleotide probing of a larger number of L. monocytogenes isolates showed that the observed differences can be used to subdivide the species. The data suggest a correspondence between the sequence type of the lisA gene and flagellar antigens. Assays based on hybridization or the polymerase chain reaction with type-specific oligonucleotides may provide fast and easy alternative methods for strain typing.
机译：已经确定了来自单核细胞增生性李斯特菌12067（一种血清型4b的人类临床分离株）的3.5-kb BamHI片段的核苷酸序列。 DNA片段包含李斯特菌溶血素基因，磷脂酰肌醇特异性磷脂酶C的一部分基因和金属蛋白酶的一部分。将该序列与来自其他两个单核细胞增生李斯特氏菌分离物的相应序列进行比较，显示出显着数量的核苷酸差异。几个差异引起氨基酸取代。可变性最大的区域是mpl基因的检测部分，而lisA基因则显示出相对较高的保守度，尤其是在氨基酸水平上。为了分析lisA基因中序列变异的模式，从36个不同来源的分离株中测序了一个覆盖9个核苷酸差异的160 bp区域。这项工作表明菌株可以根据核苷酸序列分为两种主要类型。大量单核细胞增生李斯特氏菌分离株的寡核苷酸探测显示，观察到的差异可用于细分物种。数据表明lisA基因的序列类型与鞭毛抗原之间存在对应关系。基于与类型特异性寡核苷酸的杂交或聚合酶链反应的测定法可为菌株分型提供快速简便的替代方法。

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