Isolation, expression, and nucleotide sequence of the sod gene from Porphyromonas gingivalis.

J I Choi; N Takahashi; T Kato; H K Kuramitsu

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Isolation, expression, and nucleotide sequence of the sod gene from Porphyromonas gingivalis.

机译：牙龈卟啉单胞菌sod基因的分离，表达和核苷酸序列。

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The sod gene coding for the Mn/Fe-dependent superoxide dismutase (SOD) enzyme has been isolated on a 5.9-kb DNA fragment from Porphyromonas gingivalis ATCC 53977. SOD activity can be expressed from the P. gingivalis fragment and from a subcloned fragment in Escherichia coli. However, the enzyme does not appear to be expressed from its own promoter in E. coli cells. The nucleotide sequence of the gene has been determined, and the deduced amino acid sequence of the enzyme is nearly identical to that of the enzyme purified from P. gingivalis 381 and shares extensive sequence similarity with comparable enzymes from E. coli.

机译：已经从牙龈卟啉单胞菌ATCC 53977的一个5.9-kb DNA片段上分离了编码Mn / Fe依赖的超氧化物歧化酶（SOD）酶的sod基因。SOD活性可以从牙龈卟啉单胞菌片段和亚克隆的大肠杆菌。但是，该酶在大肠杆菌细胞中似乎不是从其自身的启动子表达的。已经确定了该基因的核苷酸序列，并且推导的该酶的氨基酸序列与从齿龈假单胞菌381纯化的酶的氨基酸序列几乎相同，并且与来自大肠杆菌的可比酶具有广泛的序列相似性。

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《Infection and immunity》 |1991年第4期|共3页
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J I Choi; N Takahashi; T Kato; H K Kuramitsu;
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Isolation, expression, and nucleotide sequence of the sod gene from Porphyromonas gingivalis.

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