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首页> 外文期刊>Infection and immunity >Comparison of adherence of Pseudomonas aeruginosa to respiratory epithelial cells from cystic fibrosis patients and healthy subjects.
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Comparison of adherence of Pseudomonas aeruginosa to respiratory epithelial cells from cystic fibrosis patients and healthy subjects.

机译：铜绿假单胞菌对囊性纤维化患者和健康受试者呼吸道上皮细胞粘附的比较。

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The adherence of Pseudomonas aeruginosa PAO1 to primary cultures of cystic fibrosis nasal polyp (CFNP), normal human nasal polyp (NHNP), and immortalized CF and normal cell lines was studied. PAO1 bound significantly more to primary CFNP cells than to NHNP cells as the mean adherence +/- standard deviation of 5 x 10(7) CFU of 35S-labeled bacteria per ml per well was 15.09 x 10(6) +/- 4.25 x 10(6) CFU/ml per well and 7.62 x 10(6) +/- 2.11 x 10(6) CFU/ml per well, respectively (Mann-Whitney U test, P less than 0.0001). There was no significant difference in PAO1 adherence to the immortalized CF and normal cell lines. The primary CFNP cells had more receptors (115 per cell) than did NHNP cells (34 per cell). P. aeruginosa binding to CFNP was blocked by GlcNAc, NeuAc, L-Fuc, and D-Gal, while binding to NHNP was blocked only by GlcNAc, suggesting that receptors on the two cell types were qualitatively different. Pseudomonas supernatants containing protease, phospholipase C, and neuraminidase activity increased adherence to CFNP and NHNP cells. The Pseudomonas exoproducts modified epithelial cell glycoconjugates, as characterized by binding of fluorescein isothiocyanate-labeled lectins and the release of sialic acid. There was minimal release of fibronectin by the bacterial supernatants. The affinity of P. aeruginosa for CF epithelial cells appeared to be due to an increased number of receptors and modification of the epithelial cell surface by P. aeruginosa exoproducts that exposed asialoganglioside binding sites.
机译：研究了铜绿假单胞菌PAO1对囊性纤维化鼻息肉（CFNP），正常人鼻息肉（NHNP），永生化CF和正常细胞系原代培养物的粘附性。 PAO1与初级CFNP细胞的结合比与NHNP细胞的结合要多得多，因为每孔每毫升35S标记细菌的5 x 10（7）CFU的平均粘附性+/-标准偏差为15.09 x 10（6）+/- 4.25 x每孔10（6）CFU / ml和每孔7.62 x 10（6）+/- 2.11 x 10（6）CFU / ml（Mann-Whitney U检验，P小于0.0001）。 PAO1对永生的CF细胞和正常细胞系的依从性没有显着差异。 CFNP原代细胞比NHNP细胞（每个细胞34个）具有更多的受体（每个细胞115个）。铜绿假单胞菌与CFNP的结合被GlcNAc，NeuAc，L-Fuc和D-Gal阻断，而与NHNP的结合仅被GlcNAc阻断，这表明两种细胞类型的受体在质量上是不同的。含有蛋白酶，磷脂酶C和神经氨酸酶活性的假单胞菌上清液增加了对CFNP和NHNP细胞的粘附。假单胞菌外产物修饰上皮细胞糖缀合物，其特征在于结合异硫氰酸荧光素标记的凝集素和唾液酸的释放。细菌上清液释放的纤连蛋白极少。铜绿假单胞菌对CF上皮细胞的亲和力似乎是由于受体的数量增加和暴露去积神经节苷脂结合位点的铜绿假单胞菌外生产物对上皮细胞表面的修饰所致。

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