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首页> 外文期刊>Infection and immunity >Induction of inflammatory mediator release (serotonin and 12-hydroxyeicosatetraenoic acid) from human platelets by Pseudomonas aeruginosa glycolipid.
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Induction of inflammatory mediator release (serotonin and 12-hydroxyeicosatetraenoic acid) from human platelets by Pseudomonas aeruginosa glycolipid.

机译：铜绿假单胞菌糖脂诱导人血小板释放炎性介质（血清素和12-羟基二十碳四烯酸）。

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摘要

Purified glycolipid from Pseudomonas aeruginosa induced the generation of significant amounts of 12-hydroxyeicosatetraenoic acid (12-HETE) and serotonin release from human platelets. The release of serotonin was first observed 2 min after addition of the glycolipid and increased with time. Significant serotonin release was obtained at glycolipid concentrations above 5 micrograms/ml and increased dose-dependently up to 100% at glycolipid concentrations above 40 micrograms/ml. Glycolipid induced 12-HETE in a time- and dose-dependent manner. 12-HETE formation was first measured after 10 min of incubation and increased with time. Optimal 12-HETE formation was obtained at a glycolipid concentration of 50 micrograms/ml; higher concentrations of glycolipid led to a decrease in 12-HETE formation, indicating a cytotoxic effect. Stimulation of platelets with glycolipid (12-HETE formation and serotonin release) was accompanied by calcium influx, translocation of protein kinase C, activation of guanylylimidodiphosphate binding, and increased GTPase activity in platelet membranes within the same concentration range.
机译：来自铜绿假单胞菌的纯化糖脂诱导人血小板中大量12-羟基二十碳四烯酸（12-HETE）和5-羟色胺的释放。加入糖脂后2分钟首先观察到5-羟色胺的释放，并随时间增加。在糖脂浓度高于5微克/毫升时，血清素释放显着，在糖脂浓度高于40微克/毫升时，剂量依赖性增加至100％。糖脂以时间和剂量依赖性方式诱导12-HETE。孵育10分钟后，首先测量12-HETE的形成，并随时间增加。糖脂浓度为50微克/毫升时，可获得最佳的12-HETE形成;较高浓度的糖脂导致12-HETE形成减少，表明具有细胞毒性作用。在相同浓度范围内，用糖脂刺激血小板（12-HETE形成和5-羟色胺释放）伴随着钙的涌入，蛋白激酶C的移位，鸟苷脂二磷酸结合的激活以及GTPase活性的增加。

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