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首页> 外文期刊>Infection and immunity >Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components.
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Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components.

机译：从宿主成分中分离出的柯氏杆菌I和II期的免疫和生物学特性。

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Coxiella burnetii, phase I and II, cells cultivated in the yolk sac of chicken embryos were separated from host cell components by two cycles of isopycnic Renografin gradient centrifugation. Initial steps in the purification of viable C. burnetii involved differential centrifugation and sedimentation through an aqueous solution of 30% sucrose and 7.6% Renografin. After the first, but not the second, cycle of Renografin gradient centrifugation, the cells were passed through microfilter glass filters which facilitated the removal of host components. The integrity of morphologically different cell variants was maintained during purification procedures by suspending highly purified C. burnetii in phosphate-buffered saline-sucrose solutions. C. burnetii, phases I and II, obtained by these methods appeared to be free from host cell components by serological methods while retaining morphological integrity and infectivity for yolk sacs and experimental animals. Average yields of C. burnetii were 2.83, 1.5, and 0.84 mg (dry weight) per yolk sac of the Ohio strain (phase I), 9 Mile strain (phase I), and 9 Mile strain (phase II), respectively. Recovery of phase I cells averaged about 70%, whereas the recovery of phage II cells was approximately 40%. The temporal sequence of phase I and II antibody response was demonstrated in infected and vaccinated animals. Also, no antibody response in mice and guinea pigs to yolk sac antigens was detectable after two injections of vaccine or viable cells. Importantly, this is the first report of the separation of viable phase II cells of C. burnetii free of host components.
机译：通过等渗肾上腺素梯度离心两个周期，将鸡胚卵黄囊中培养的柯氏杆菌I和II期细胞与宿主细胞成分分离。纯化活的梭状芽胞杆菌的初始步骤包括通过30％蔗糖和7.6％肾上腺素的水溶液进行不同程度的离心和沉淀。在肾上腺素梯度离心的第一个而不是第二个循环之后，使细胞通过微滤器玻璃滤器，该滤器有助于去除宿主成分。在纯化过程中，通过将高度纯化的伯氏梭菌悬浮在磷酸盐缓冲液-蔗糖溶液中，可以保持形态不同的细胞变异体的完整性。通过血清学方法，通过这些方法获得的伯氏梭菌I期和II期似乎不含宿主细胞成分，同时保留了卵黄囊和实验动物的形态完整性和感染性。俄亥俄菌株（I期），9英里菌株（I期）和9英里菌株（9期菌株）的卵黄囊的平均产量分别为2.83、1.5和0.84 mg（干重）。 I期细胞的回收率平均约为70％，而噬菌体II细胞的回收率约为40％。在感染和接种疫苗的动物中证明了I和II期抗体反应的时间顺序。同样，在两次注射疫苗或活细胞后，在小鼠和豚鼠中对卵黄囊抗原没有检测到抗体反应。重要的是，这是分离不含宿主成分的伯氏梭状芽胞杆菌可行的II期细胞的第一份报告。

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