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PNA Pepetide nucleic acid probe for identifying Coxiella burnetii and Method for identifying Coxiella burnetii using the same
PNA Pepetide nucleic acid probe for identifying Coxiella burnetii and Method for identifying Coxiella burnetii using the same
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机译:用于鉴定伯氏柯氏杆菌的pna肽核酸探针和使用该探针的鉴定伯氏柯氏杆菌的方法
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摘要
The present invention relates to a peptide nucleic acid (PNA) probe for detecting Coxiella burnetii and a Coxiella burnetii detection method using the same. More specifically, according to the present invention, a Coxiella burnetii gene is amplified by using a PNA probe represented as a base sequence selected from groups consisting of sequence numbers 4 to 6, and a primer represented as the sequence numbers 1 and 3. The amplified gene is hybridized with the PNA probe, and Coxiella burnetii is detected through a melting curve analysis of the hybridized material. Accordingly, the PNA probe can detect Coxiella burnetii, which is a cause bacillus of Q-fever, at higher sensitivity and correctness than that of a conventional polymerase chain reaction (PCR) method, and can easily discriminate false positiveness, thereby being useful for Q-fever diagnosis.
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