Screening of a recombinant mycobacterial DNA library with polyclonal antiserum and molecular weight analysis of expressed antigens.

D B Young; L Kent; R A Young

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Screening of a recombinant mycobacterial DNA library with polyclonal antiserum and molecular weight analysis of expressed antigens.

机译：用多克隆抗血清筛选重组分枝杆菌DNA文库，并对表达的抗原进行分子量分析。

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A lambda gt11 expression library containing recombinant DNA from Mycobacterium tuberculosis was screened using hyperimmune anti-M. tuberculosis rabbit serum. The majority (22 of 29) of the recombinant clones selected by using polyclonal serum expressed three antigens that were previously identified by using mouse monoclonal antibodies, thus indicating the immunodominance of these proteins. Western blot analysis of the recombinant clones demonstrated that expression of these antigens is frequently independent of the formation of beta-galactosidase fusion proteins. The molecular weight of each expressed antigen can vary between clones and is not necessarily identical to that found in mycobacterial extracts.

机译：使用超免疫抗-M筛选包含结核分枝杆菌重组DNA的λgt11表达文库。结核兔血清。使用多克隆血清选择的大多数重组克隆（29个中的22个）表达了三种抗原，这些抗原先前已使用小鼠单克隆抗体进行了鉴定，从而表明了这些蛋白的免疫优势。重组克隆的蛋白质印迹分析表明，这些抗原的表达通常独立于β-半乳糖苷酶融合蛋白的形成。每个表达的抗原的分子量可以在克隆之间变化，并且不一定与在分枝杆菌提取物中发现的分子量相同。

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《Infection and immunity》 |1987年第6期|共5页
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D B Young; L Kent; R A Young;
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中图分类医学免疫学;
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6. Screening of a recombinant mycobacterial DNA library with polyclonal antiserum and molecular weight analysis of expressed antigens. [O] . D B Young, L Kent, R A Young 1987

机译：用多克隆抗血清筛选重组分枝杆菌DNA文库并对表达的抗原进行分子量分析。
7. Highly efficient immunodiagnosis of Large cardamom chirke virus using the polyclonal antiserum against Escherichia coli expressed recombinant coat protein [O] . S. Vijayanandraj, M. Yogita, Amrita Das, 2013

机译：使用多克隆抗血管对大肠杆菌的大肠杆菌抗血管进行高效免疫诊断，对大肠杆菌的COLI表达重组涂层蛋白质
8. Improved Method for Screening cDNA Expression Libraries for DNA-Binding Proteins [R] . Harline, Kandala, Sage, 1992

机译：筛选DNa结合蛋白cDNa表达文库的改进方法

Screening of a recombinant mycobacterial DNA library with polyclonal antiserum and molecular weight analysis of expressed antigens.

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