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首页> 外文期刊>Infection and immunity >Intracellular spread of Shigella flexneri associated with the kcpA locus and a 140-kilodalton protein.
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Intracellular spread of Shigella flexneri associated with the kcpA locus and a 140-kilodalton protein.

机译：弗氏志贺氏菌在细胞内的传播与kcpA基因座和140千达尔顿蛋白有关。

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Escherichia coli K-12 hybrids carrying both the 220-kilobase plasmid and the purE-linked kcpA locus from Shigella flexneri expressed a 140-kilodalton (kDa) protein which was recognized by convalescent sera from monkeys infected with S. flexneri. These hybrids were tested for the ability to produce plaques in HeLa cell monolayers. Hybrid strains which carried both the 220-kilobase plasmid and the kcpA locus had a plaque-forming efficiency of at least 10(-4) PFU/CFU, whereas the plaque-forming efficiency of hybrids that carried only the shigella invasion plasmid ranged from undetectable to 10(-6). Variants were purified from the rare plaques formed by E. coli hybrids that carried only the shigella invasion plasmid. These plaque-purified strains also expressed the 140-kDa protein, and they had a plaque-forming efficiency of at least 10(-4). Transduction of the purE locus from a plaque-purified hybrid into a non-plaque-forming E. coli K-12 strain did not alter the phenotype of the recipient, but conjugation of the shigella invasion plasmid into this transductant reconstituted both expression of the 140-kDa protein and the plaque-forming phenotype. Invasive E. coli K-12 hybrids carrying only the shigella invasion plasmid remained localized within discrete areas of the HeLa cell cytoplasm, whereas hybrids that also carried the S. flexneri kcpA locus grew in a dispersed pattern throughout the host cell cytoplasm. The dispersal of these organisms was inhibited by cytochalasin D, which suggested that host cell microfilaments may play a role in the intracellular spread of enteroinvasive pathogens.
机译：带有220碱基碱基的质粒和来自弗氏志贺氏菌的purE连接的kcpA基因座的大肠杆菌K-12杂种表达了140千道尔顿（kDa）蛋白，该蛋白可以被感染弗氏链球菌的猴子的恢复期血清识别。测试了这些杂种在HeLa细胞单层中产生噬菌斑的能力。同时携带220碱基碱基的质粒和kcpA基因座的杂种菌株的噬斑形成效率至少为10（-4）PFU / CFU，而仅携带志贺氏菌入侵质粒的杂种的噬菌斑形成效率介于无法检测的范围内至10（-6）。从仅携带志贺氏菌入侵质粒的大肠杆菌杂种形成的罕见噬菌斑中纯化变体。这些噬菌斑纯化的菌株还表达了140-kDa的蛋白质，它们的噬菌斑形成效率至少为10（-4）。将purE基因座从噬菌斑纯化的杂种中转导到非噬菌斑形成的大肠杆菌K-12菌株中，不会改变受体的表型，但志贺氏菌入侵质粒与该转导子的结合重新构建了140的表达-kDa蛋白和噬斑形成表型。仅携带志贺氏菌入侵质粒的侵袭性大肠杆菌K-12杂种保持定位在HeLa细胞质的离散区域内，而同时也携带弗氏链球菌kcpA基因座的杂种则在整个宿主细胞质中以分散的模式生长。这些生物的扩散受到细胞松弛素D的抑制，这表明宿主细胞微丝可能在肠道侵袭性病原体的细胞内扩散中发挥作用。

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