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首页> 外文期刊>Infection and immunity >Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli.
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Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli.

机译：大肠杆菌定居因子抗原I基因的分子克隆和核苷酸序列。

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The colonization factor antigen I (CFA/I) gene has been isolated and sequenced. The amino acid sequence of CFA/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA/I protein since they are not present in the protein sequence. Among the remaining amino acids, only two are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA/I gene promoter. No sequences homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of eight A's are located 45 bp downstream from the termination codon of the CFA/I gene; this region may be a rho-independent transcriptional terminator.
机译：殖民因子抗原I（CFA / I）基因已被分离和测序。由核苷酸序列推导的CFA / I的氨基酸序列由170个氨基酸组成。前23个氨基酸被认为是CFA / I蛋白的信号肽，因为它们不存在于蛋白序列中。在其余氨基酸中，只有两个与蛋白质序列不同：氨基酸第76位是天冬氨酸而不是天冬酰胺，而第97位是丝氨酸而不是丙氨酸。 CFA / I基因具有一个典型的Shine-Dalgarno序列，位于起始密码子上游10个碱基对（bp）处。暂定起始密码子上游48 bp处的序列TACAAT暂定为CFA / I基因启动子的-10序列。没有发现与共有-35启动子序列同源的序列。一对反向重复序列，随后延伸了八个A，位于CFA / I基因的终止密码子下游45 bp。该区域可以是不依赖rho的转录终止子。

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