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首页> 外文期刊>Infection and immunity >Evidence that the serotype b antigenic determinant of Actinobacillus actinomycetemcomitans Y4 resides in the polysaccharide moiety of lipopolysaccharide.
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Evidence that the serotype b antigenic determinant of Actinobacillus actinomycetemcomitans Y4 resides in the polysaccharide moiety of lipopolysaccharide.

机译：放线杆菌放线放线杆菌Y4的血清型b抗原决定簇存在于脂多糖的多糖部分中的证据。

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A high-molecular-weight polysaccharide-containing antigen was isolated from a phenol-water extract of Actinobacillus actinomycetemcomitans ATCC 43718 (formerly Y4) by gel permeation chromatography in lipopolysaccharide (LPS)-disaggregating buffer. The polysaccharide antigen formed a precipitin band with rabbit serotype b-specific antiserum but not with rabbit antisera to serotype a or c. Electroblotted serotype b antigen was probed with serum from a patient with localized juvenile periodontitis (LJP), resulting in a diffuse "smear" in the upper region of the lane. By utilizing an enzyme-linked immunosorbent assay, it was demonstrated that the geometric mean immunoglobulin G antibody titer to the serotype b polysaccharide was significantly higher in sera from LJP patients than in sera from periodontally healthy individuals. Moreover, LJP antibody titers to the serotype b polysaccharide exhibited age-dependent variation. Double immunodiffusion analysis revealed that the serotype b antigen formed a line of identity with low-molecular-weight LPS following reaction with serotype b-specific antiserum. Incubation of LJP serum in the presence of a lipid-free polysaccharide moiety obtained by mild acid hydrolysis of LPS from A. actinomycetemcomitans Y4 markedly reduced immunoglobulin G titer to the serotype b antigen. In contrast, solubilized lipid A was only weakly inhibitory. The results of this study indicate that the serotype b-specific determinant of A. actinomycetemcomitans resides in the polysaccharide moiety of LPS and represents a major target for immunoglobulin G antibody in serum of LJP subjects colonized by this organism.
机译：通过脂多糖（LPS）分解缓冲液中的凝胶渗透色谱法，从放线放线放线杆菌ATCC 43718（以前为Y4）的苯酚-水提取物中分离出一种含高分子量多糖的抗原。多糖抗原与兔血清型b特异性抗血清形成了沉淀蛋白条带，但与兔血清型a或c没有形成沉淀蛋白带。用局部少年牙周炎（LJP）患者的血清探测电印迹的血清型b抗原，导致泳道上部区域弥漫性“涂片”。通过利用酶联免疫吸附测定，证明了来自LJP患者的血清中针对血清B型多糖的几何平均免疫球蛋白G抗体滴度明显高于来自牙周健康个体的血清中。此外，针对血清型b多糖的LJP抗体效价显示出年龄依赖性变化。双重免疫扩散分析显示，在与血清型b特异性抗血清反应后，血清型b抗原与低分子量LPS形成了同一性。 LJP血清的培养是在无脂多糖部分的存在下进行的，该部分是由APS放线菌Y4轻度酸水解LPS获得的LPS显着降低的免疫球蛋白G滴度至血清型b抗原。相反，增溶的脂质A仅具有弱抑制性。这项研究的结果表明，放线放线杆菌的血清型b特异性决定子位于LPS的多糖部分，并代表该微生物定植的LJP受试者血清中免疫球蛋白G抗体的主要靶标。

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