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首页> 外文期刊>Infection and immunity >Immunochemical and structural characterization of a serotype-specific polysaccharide antigen from Actinobacillus actinomycetemcomitans Y4 (serotype b).
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Immunochemical and structural characterization of a serotype-specific polysaccharide antigen from Actinobacillus actinomycetemcomitans Y4 (serotype b).

机译：放线放线杆菌Y4（血清型b）的血清型特异性多糖抗原的免疫化学和结构表征。

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摘要

A serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b) was extracted from whole cells by autoclaving. The extract was purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300 columns. The purified polysaccharide antigen formed a single precipitin line with anti-type b serum but not with anti-type a serum and anti-type c serum. The antigen was composed of 43.9% L-rhamnose, 49.1% D-fucose, and a trace amount of fatty acid. Methylation analysis, Smith degradation, and optical rotation data showed that the antigen was a polymer consisting of a disaccharide repeating unit, ----3)-alpha-D-fucopyranosyl-(1----2)-beta-L-rhamnopyranosyl-(1----. In quantitative precipitin inhibition tests, D-fucose and L-rhamnose showed very low inhibition, but the partial hydrolysate of the purified antigen was an effective inhibitor, suggesting that the serotype b specific antiserum recognizes the larger oligosaccharide units.
机译：通过高压灭菌从全细胞中提取放线放线杆菌放线放线杆菌Y4的血清型特异性多糖抗原（血清型b）。通过在DEAE-Sephadex A-25和Sephacryl S-300柱上的色谱法纯化提取物。纯化的多糖抗原与抗b型血清形成单一的沉淀素系，但与抗a型血清和抗c型血清则没有。抗原由43.9％的L-鼠李糖，49.1％的D-岩藻糖和微量的脂肪酸组成。甲基化分析，Smith降解和旋光度数据表明，该抗原是由双糖重复单元----- 3）-α-D-呋喃果糖基-（1 ---- 2）-β-L-组成的聚合物鼠李糖吡喃糖基-（1 ----。在定量沉淀蛋白抑制试验中，D-岩藻糖和L-鼠李糖显示出极低的抑制作用，但纯化抗原的部分水解产物是有效的抑制剂，表明血清型b特异性抗血清可识别更大的寡糖单元。

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