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首页> 外文期刊>Infection and immunity >A recombinant Salmonella typhimurium vaccine induces local immunity by four different routes of immunization.
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A recombinant Salmonella typhimurium vaccine induces local immunity by four different routes of immunization.

机译：重组鼠伤寒沙门氏菌疫苗通过四种不同的免疫途径诱导局部免疫。

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Immunization of mice with an attenuated Salmonella typhimurium strain (Phopc) carrying a plasmid encoding a hybrid form of the hepatitis B virus core antigen (HBc) induced specific antibody responses against the bacterial lipopolysaccharide (LPS) and HBc. Different mucosal routes of immunization, i.e., oral, nasal, rectal, and vaginal, were compared for their ability to induce a systemic as well as a mucosal response at sites proximal or distant to the site of immunization. Anti-LPS and anti-HBc immunoglobulin A (IgA) antibodies were measured in saliva, in feces, and in genital, bronchial, and intestinal secretions. Specific antibodies in serum and secretions were observed after immunization via all routes; however, the response to LPS was independent of that against HBc. In serum, saliva, and genital and bronchial secretions, high amounts of anti-HBc IgA were obtained by the nasal route of immunization. Vaginal immunization resulted in two different responses in mice: high and low. We observed a correlation between the level of specific immune response and the estrous status of these mice at the time of immunization. Rectal immunization induced high amounts of IgA against HBc and LPS in colonorectal secretions and feces but not at distant sites. These data suggest that S. typhimurium is able to invade different mucosal tissues and induce long-lasting local IgA responses against itself and a carried antigen after a single immunization.
机译：用携带编码乙型肝炎病毒核心抗原（HBc）杂合形式的质粒的减毒鼠伤寒沙门氏菌菌株（Phopc）免疫小鼠，可诱导针对细菌脂多糖（LPS）和HBc的特异性抗体反应。比较了不同的粘膜免疫途径，即口服，鼻，直肠和阴道，在邻近或远离免疫部位的部位诱导全身性和粘膜应答的能力。在唾液，粪便以及生殖器，支气管和肠道分泌物中测量了抗LPS和抗HBc免疫球蛋白A（IgA）抗体。通过所有途径免疫后观察到血清和分泌物中的特异性抗体。但是，对LPS的反应独立于对HBc的反应。在血清，唾液以及生殖器官和支气管分泌物中，通过鼻免疫途径获得了大量的抗HBc IgA。阴道免疫在小鼠中产生两种不同的反应：高和低。我们观察到特异性免疫应答的水平与这些小鼠在免疫时的发情状态之间的相关性。直肠免疫可在结肠直肠分泌物和粪便中诱导出针对HBc和LPS的大量IgA，但在远处却没有。这些数据表明，鼠伤寒沙门氏菌能够侵入不同的粘膜组织，并在单次免疫后诱导针对其自身和携带的抗原的持久性局部IgA反应。

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