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首页> 外文期刊>Infection and immunity >Proteus mirabilis ambient-temperature fimbriae: cloning and nucleotide sequence of the aft gene cluster.
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Proteus mirabilis ambient-temperature fimbriae: cloning and nucleotide sequence of the aft gene cluster.

机译：变形杆菌环境温度菌毛：后部基因簇的克隆和核苷酸序列。

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Uropathogenic Proteus mirabilis produces at least four types of fimbriae. Amino acid sequences from two peptides, derived by tryptic digestion of the structural subunit of one type of these fimbriae, the ambient-temperature fimbriae, were determined: NVVPGQPSSTQ and LIEGENQLNYNA. PCR primers, based on these sequences and that of the N terminus, were used to amplify a 359-bp fragment. A cosmid clone, isolated from a P. mirabilis genomic library by hybridization with the 359-bp PCR product, was used to determine the nucleotide sequence of the atf gene cluster. A 3,903-bp region encodes three polypeptides: AtfA, the structural subunit; AtfB, the chaperone; and AtfC, the outer membrane molecular usher. No fimbria-related genes are evident either 5' or 3' to the three contiguous genes. AtfA demonstrates significant amino acid sequence identity with type 1 major fimbrial subunits of several enteric species. The 359-bp PCR product hybridized strongly with all Proteus isolates (n = 9) and 25% of 355 Escherichia coli isolates but failed to hybridize with any of 26 isolates among nine other uropathogenic species. Ambient-temperature fimbriae of P. mirabilis may represent a novel type of fimbriae of enteric species.
机译：泌尿致病性变形杆菌产生至少四种类型的菌毛。确定了来自两种肽的氨基酸序列，这些蛋白是通过胰蛋白酶消化这些菌毛中的一种（环境温度菌毛）的结构亚基而得到的：NVVPGQPSSTQ和LIEGENQLNYNA。基于这些序列和N端的PCR引物可扩增359 bp片段。通过与359-bp PCR产物杂交从黏液毕赤酵母基因组文库中分离出的粘粒克隆用于确定atf基因簇的核苷酸序列。一个3,903 bp的区域编码三种多肽：AtfA，结构亚基; AtfB，伴侣;和AtfC，外膜分子的引入。三个连续基因的5'或3'均未发现与菌毛相关的基因。 AtfA证明与几种肠溶菌种的1型主要纤维亚基具有显着的氨基酸序列同一性。 359 bp PCR产物与所有Proteus分离株（n = 9）和355种大肠杆菌分离株中的25％强烈杂交，但未能与其他9种尿毒症物种中的26种分离株杂交。奇异假单胞菌的环境温度菌毛可能代表了一种新型的肠溶菌菌毛。

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