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首页> 外文期刊>Infection and immunity >Role of coagulase in a murine model of hematogenous pulmonary infection induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads.
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Role of coagulase in a murine model of hematogenous pulmonary infection induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads.

机译：凝血酶在鼠静脉内注射金黄色葡萄球菌所致的血源性肺部感染鼠模型中的作用。

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We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of bacteria, including three strains of S. aureus, two strains of Staphylococcus epidermidis, one strain of Streptococcus pyogenes, three strains of Pseudomonas aeruginosa, and one strain of Klebsiella pneumoniae. All except two strains of S. aureus were cleared rapidly from the lungs. When S. aureus NUMR1 enmeshed in agar beads was injected intravenously, the organisms concentrated and remained in the lung for a period longer than several weeks. Multiple lung abscesses were evident macroscopically, and histological examination of the infected lung showed multiple lung abscesses around the pulmonary arterioles, consisting of bacterial colonies encircled with fibrin filaments and surrounded by inflammatory cells of neutrophils and macrophages. When 14 strains of clinically isolated S. aureus were injected intravenously, the number of bacteria recovered from the lung tissue 7 days after infection correlated with the titer of staphylocoagulase (P < 0.01) but not with the titer of clumping factor. Injection of coagulase-deficient mutant strain DU5843 was associated with a markedly reduced number of viable bacteria isolated from the lung, compared with its coagulase-positive parental strain DU5789. Our results suggest that coagulase may play a role in the development of blood-borne staphylococcal pneumonia in our model. Our animal model is simple and reproducible and resembles blood-borne staphylococcal pneumonia in humans, and it could be useful for investigating the pathogenicity or treatment of staphylococcal pulmonary infection, including infections with methicillin-resistant S. aureus.
机译：我们描述了一种急性小鼠葡萄球菌性肺炎的新型小鼠模型，该模型由静脉注射金黄色葡萄球菌浸入琼脂珠中诱导。为了进行比较，我们还使用了各种细菌菌株，包括三株金黄色葡萄球菌，两株表皮葡萄球菌，一株化脓性链球菌，三株铜绿假单胞菌和一株肺炎克雷伯菌。除两株金黄色葡萄球菌外，所有其他细菌均已从肺中迅速清除。静脉内注射琼脂珠粒中的金黄色葡萄球菌NUMR1时，该生物体浓缩并在肺中保留了超过几周的时间。肉眼可见多处肺脓肿，受感染的肺组织学检查显示，肺小动脉周围有多处肺脓肿，由细菌菌落和纤维蛋白丝包围，并被中性粒细胞和巨噬细胞的炎性细胞包围。当静脉注射14株临床分离的金黄色葡萄球菌时，感染后7天从肺组织中回收的细菌数量与葡萄球菌凝固酶的滴度相关（P <0.01），而与聚集因子的滴度无关。与凝固酶阳性的亲本菌株DU5789相比，凝固酶缺陷型突变菌株DU5843的注射与从肺中分离出的活菌数量明显减少有关。我们的结果表明，在我们的模型中，凝固酶可能在血源性葡萄球菌性肺炎的发生中起作用。我们的动物模型简单且可重现，类似于人类血源性葡萄球菌性肺炎，可用于研究葡萄球菌肺部感染（包括耐甲氧西林金黄色葡萄球菌的感染）的致病性或治疗。

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