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首页> 外文期刊>Infection and immunity >Variable number of tandem repeats in clinical strains of Haemophilus influenzae.
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Variable number of tandem repeats in clinical strains of Haemophilus influenzae.

机译：流感嗜血杆菌临床株中串联重复序列的数目可变。

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An algorithm capable of identifying short repeat motifs was developed and used to screen the whole genome sequence available for Haemophilus influenzae, since some of these repeats have been shown to affect bacterial virulence. Various di- to hexanucleotide repeats were identified, confirming and extending previous findings on the existence of variable-number-of-tandem-repeat loci (VNTRs). Repeats with units of 7 or 8 nucleotides were not encountered. For all of the 3- to 6-nucleotide repeats in the H. influenzae chromosome, PCR tests capable of detecting allelic polymorphisms were designed. Fourteen of 18 of the potential VNTRs were indeed highly polymorphic when different strains were screened. Two of the potential VNTRs appeared to be short and homogeneous in length; another one may be specific for the H. influenzae Rd strain only. One of the primer sets generated fingerprint-type DNA banding patterns. The various repeat types differed with respect to intrinsic stability as well. It was noted for separate colonies derived from a single clinical specimen or strains passaged for several weeks on chocolate agar plates that the lengths of the VNTRs did not change. When several strains from different patients infected during an outbreak of lung disease were analyzed, increased but limited variation was encountered in all VNTR sites analyzed. One of the 5-nucleotide VNTRs proved to be hypervariable. This variability may reflect the molecular basis of a mechanism used by H. influenzae bacteria to successfully colonize and infect different human individuals.
机译：能够识别短重复序列基序的算法已经开发出来，并用于筛选可用于流感嗜血杆菌的整个基因组序列，因为这些重复序列中的某些已显示出会影响细菌的毒力。鉴定了各种二至六核苷酸重复，证实并扩展了先前关于可变数目的串联重复基因座（VNTR）的发现。没有遇到以7或8个核苷酸为单位的重复。对于流感嗜血杆菌染色体中的所有3至6个核苷酸重复，设计了能够检测等位基因多态性的PCR试验。当筛选不同的菌株时，潜在的18种VNTR中有14种确实高度多态。潜在的VNTR中有两个看起来很短且长度均一。另一种可能仅针对流感嗜血杆菌Rd株。其中一组引物产生了指纹型DNA条带图谱。各种重复类型在固有稳定性方面也有所不同。注意到对于源自单个临床标本的单独菌落或在巧克力琼脂平板上传代数周的菌株，VNTR的长度没有改变。当分析来自在肺疾病暴发期间感染的不同患者的几种菌株时，在所有分析的VNTR位点中变异均增加，但变化有限。 5-核苷酸VNTR之一被证明是高变的。这种变异性可能反映了流感嗜血杆菌细菌成功定居并感染不同人类个体的机制的分子基础。

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