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首页> 外文期刊>Infection and immunity >Murine Immune Responses to Neisseria meningitidis Group C Capsular Polysaccharide and a Thymus-Dependent Toxoid Conjugate Vaccine
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Murine Immune Responses to Neisseria meningitidis Group C Capsular Polysaccharide and a Thymus-Dependent Toxoid Conjugate Vaccine

机译：脑膜炎奈瑟菌C组荚膜多糖和胸腺依赖类毒素共轭疫苗的小鼠免疫反应。

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The polysaccharide (PS) capsules of many pathogenic bacteria are poor immunogens in infants and young children as a result of the delayed response to PS antigens during ontogeny. The development of polysaccharide-protein conjugate vaccines for Haemophilus influenzae type b, which have proven to be efficacious in this age group, has led to active development by a number of investigators of conjugate vaccines for other diseases. We describe here the response of several mouse strains to the capsular PS of Neisseria meningitidis group C (MCPS) conjugated to tetanus toxoid (MCPS-TT) and the same response in BALB/c mice as a model of the immune consequences of conjugate vaccine immunization. The use of a conjugate vaccine results in a shift in the isotype elicited in response to the MCPS, from immunoglobulin M (IgM) and IgG3 to primarily IgG1. A response to MCPS-TT is seen even among mouse strains which respond poorly to MCPS itself, emphasizing the importance of a strain survey when choosing a mouse model for a vaccine. The marked increase in IgG1 antibody titer was accompanied by a large increase in bactericidal activity of sera from these animals. Animals primed with the conjugate vaccine demonstrated a booster response after secondary immunization with either the MCPS or the conjugate. The ability to produce a boosted IgG1 anti-MCPS response to the MCPS can be transferred to adoptive recipients by B cells alone from mice primed with MCPS-TT but not mice primed with MCPS alone. These data indicate that in BALB/c mice a single immunization with MCPS-TT is sufficient to induce a shift to IgG1 and generate a memory B-cell population that does not require T cells for boosting.
机译：由于个体发育过程中对PS抗原的反应延迟，许多致病细菌的多糖（PS）胶囊在婴儿和幼儿中免疫原性较差。已证明在该年龄组中有效的b型流感嗜血杆菌多糖蛋白结合疫苗的开发已引起许多研究者针对其他疾病的结合疫苗的积极开发。我们在这里描述了几种小鼠品系对脑膜炎奈瑟氏球菌C组（MCPS）结合破伤风类毒素（MCPS-TT）的荚膜PS的反应，以及在BALB / c小鼠中作为相同模型的相同反应结合疫苗免疫的免疫后果。结合疫苗的使用导致响应MCPS引起的同种型从免疫球蛋白M（IgM）和IgG3转变为主要的IgG1。即使在对MCPS本身反应较差的小鼠品系中也看到了对MCPS-TT的反应，从而在选择疫苗的小鼠模型时强调了品系调查的重要性。 IgG1抗体效价的显着提高伴随着这些动物血清的杀菌活性的大幅提高。用MCPS或结合物二次免疫后，接种结合物疫苗的动物表现出加强反应。产生增强的针对MCPS的IgG1抗MCPS应答的能力可以通过仅B细胞从用MCPS-TT引发的小鼠转移到过继受体，而不能用仅用MCPS引发的小鼠转移给过继受体。这些数据表明，在BALB / c小鼠中，用MCPS-TT进行单次免疫足以诱导向IgG1的转移，并产生不需要T细胞加强免疫力的记忆B细胞群。

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