Phage Antibodies Obtained by Competitive Selection on Complement-Resistant Moraxella (Branhamella)catarrhalis Recognize the High-Molecular-Weight Outer Membrane Protein

Edwin Boel; Hester Bootsma; John de Kruif; Margriet Jansze; Karin L. Klingman; Hans van Dijk; Ton Logtenberg

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Phage Antibodies Obtained by Competitive Selection on Complement-Resistant Moraxella (Branhamella)catarrhalis Recognize the High-Molecular-Weight Outer Membrane Protein

机译：通过竞争选择抗补体莫拉氏菌（Branhamella）卡他类获得的噬菌体抗体识别高分子量外膜蛋白。

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We used competitive panning to select a panel of 10 different human antibodies from a large semisynthetic phage display library that distinguish between serum complement-resistant and complement-sensitive strains of the gram-negative diplococcus Moraxella(Branhamella) catarrhalis. Western blotting analyses and inhibition enzyme-linked immunosorbent assays showed that all phage antibodies were directed against the same or closely spaced epitopes on the target protein, which is the high-molecular-weight outer membrane protein (HMW-OMP) of M. catarrhalis. HMW-OMP was found in multiple isolates of complement-resistant but not complement-sensitive M. catarrhalis strains. Nucleotide sequence analysis demonstrated that the immunoglobulin heavy- and light-chain variable-region genes encoding the 10 phage antibodies were remarkably similar, with a strong preference for basic amino acid residues in the heavy-chain CDR3 regions. This is the first report showing that competitive panning is a successful procedure to obtain phage antibodies against differentially expressed structures on phenotypically dissimilar strains of prokaryotic cells.

机译：我们使用竞争性淘选从大型半合成噬菌体展示文库中选择了10种不同的人类抗体，以区分革兰氏阴性双球菌莫拉克氏菌（ Branhamella ）卡他氏菌。 Western印迹分析和抑制酶联免疫吸附试验表明，所有噬菌体抗体均针对目标蛋白上的相同或紧密间隔的表位，这是 M的高分子量外膜蛋白（HMW-OMP）。卡他性炎。 HMW-OMP被发现具有多个抗补体但对补体敏感的 M分离株。卡他氏菌菌株。核苷酸序列分析表明，编码10个噬菌体抗体的免疫球蛋白重链和轻链可变区基因非常相似，其中重链CDR3区的碱性氨基酸残基具有很高的偏好。这是第一份表明竞争性淘选是成功的方法，用于获得针对原核细胞表型不同的菌株上差异表达的结构的噬菌体抗体。 展开▼

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《Infection and immunity》 |1998年第1期|共6页

作者
Edwin Boel; Hester Bootsma; John de Kruif; Margriet Jansze; Karin L. Klingman; Hans van Dijk; Ton Logtenberg;
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中图分类医学免疫学;

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1. Purification and characterization of a high-molecular-weight outer membrane protein of Moraxella (Branhamella) catarrhalis. [J] . K L Klingman, T F Murphy Infection and immunity . 1994,第4期

机译：卡他莫拉菌（Branhamella）的高分子量外膜蛋白的纯化和鉴定。

2. Serum antibody response to outer membrane proteins of Moraxella (Branhamella) catarrhalis in patients with bronchopulmonary infection. [J] . J J Christensen, N Q Hansen, B Bruun Clinical and diagnostic laboratory immunology . 1996,第6期

机译：支气管肺部感染患者对卡他莫拉菌（Branhamella）的外膜蛋白的血清抗体反应。

3. Outer membrane protein B1, an iron-repressible protein conserved in the outer membrane of Moraxella (Branhamella) catarrhalis, binds human transferrin. [J] . A A Campagnari, T F Ducey, C A Rebmann Infection and immunity . 1996,第9期

机译：外膜蛋白B1是卡他莫拉菌（Branhamella）卡他拉菌外膜中保守的铁可抑制蛋白，结合人转铁蛋白。

4. Selection of Monoclonal Antibodies Targeting Therapeutic Membrane Proteins Through a Cell-Based Phage Display Panning System [C] . Yu Jung Kim, Bin Yoo, Yoonji Kim, Protein Engineering Summit. . 2018

机译：通过基于细胞的噬菌体展示平移系统靶向治疗膜蛋白的单克隆抗体的选择

5. Molecular characterization of outer membrane protein E of Moraxella (Branhamella) catarrhalis. [D] . Bhushan, Reva. 1995

机译：卡他莫拉菌（Branhamella）的外膜蛋白E的分子表征。

6. Phage Antibodies Obtained by Competitive Selection on Complement-Resistant Moraxella (Branhamella) catarrhalis Recognize the High-Molecular-Weight Outer Membrane Protein [O] . Edwin Boel, Hester Bootsma, John de Kruif, 1998

机译：通过竞争选择抗补体莫拉氏菌（布拉汉杆菌）获得的噬菌体抗体识别高分子量外膜蛋白。

7. Phage Antibodies Obtained by Competitive Selection on Complement-Resistant Moraxella ( Branhamella ) catarrhalis Recognize the High-Molecular-Weight Outer Membrane Protein [O] . Edwin Boel, Hester Bootsma, John de Kruif, 1998

机译：通过抗性静脉（Branhamella）Catarrhalis竞争选择获得的噬菌体抗体识别高分子量外膜蛋白

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2. 肺结核继发卡他莫拉氏菌感染58例临床分析 [J] . 邓立宏 ,刘东洋 . 中国防痨杂志 . 1999,第004期

3. 卡他莫拉氏菌在下呼吸道感染中的发病情况和对抗生素的敏感性 [J] . 何巧洁 ,薛欣 . 大连医学院学报 . 1994,第004期

4. 淋病奈瑟氏菌、卡他莫拉(布兰汉)氏菌及其药物敏感试验 [J] . 陈秀枢 . 临床检验杂志 . 1992,第004期

5. 应用噬菌体随机肽库研究抗溶藻弧菌及其外膜蛋白单抗识别的特异性表位 [J] . 黄志坚 ,何建国 . 中国学术期刊文摘 . 2007,第014期

6. 卡他莫拉菌UspA1来源重组蛋白rD-7对人单核细胞分化及DC成熟的影响研究 [A] . 解奇 . 2014

1. 基于卡他莫拉菌表面蛋白抗体的卡他莫拉菌Elisa检测试剂盒及制备方法 [P] . 中国专利： CN110540596B . 2021.04.06

2. 基于卡他莫拉菌表面蛋白抗体的卡他莫拉菌Elisa检测试剂盒及制备方法 [P] . 中国专利： CN110540596A . 2019-12-06

3. METHOD OF ISOLATION AND PURIFICATION OF PROTEIN OF MORAXELLA CATARRHALIS EXTERNAL MEMBRANE, STRAIN MORAXELLA CATARRHALIS FOR PREPARING PROTEIN CD, ISOLATED AND PURIFIED NONDENATURATED PROTEIN CD OF MORAXELLA CATARRHALIS EXTERNAL MEMBRANE AND IMMUNOGENIC COMPOSITION COMPRISING PROTEIN CD OF MORAXELLA CATARRHALIS EXTERNAL MEMBRANE [P] . 外国专利： RU2186582C2 . 2002-08-10

机译：分离和纯化毛MOR膜外膜蛋白的方法，应变毛MOR膜蛋白的制备，分离和纯化的毛AX壳膜外膜内膜和免疫原性复合蛋白的未变性蛋白质CD

4. PROTEINS, PEPTIDES AND OLIGOPEPTIDES OF THE OUTER MEMBRANE PROTEIN " CD" OF BRANHAMELLA CATARRHALIS, NUCLEIC ACIDS ENCODING THEM, METHOD FOR THEIR PREPARATION AND USE THEREOF AS VACCINE FORMULATIONS [P] . 外国专利： IL111102B . 2005-12-18

机译：毛状分支杆菌外膜蛋白“ CD”的蛋白质，肽和寡肽，编码它们的核酸，其制备方法和用作疫苗制剂的方法

5. Proteins, peptides and oligopeptides of the outer membrane protein "cd" of branhamella catarrhalis, nucleic acids encoding them, method for their preparation and use thereof as vaccine formulations [P] . 外国专利： IL111102A . 2005-12-18

机译：卡他氏杆菌的外膜蛋白“ cd”的蛋白质，肽和寡肽，编码它们的核酸，其制备方法及其作为疫苗制剂的用途

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Phage Antibodies Obtained by Competitive Selection on Complement-Resistant Moraxella (Branhamella)catarrhalis Recognize the High-Molecular-Weight Outer Membrane Protein

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