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首页> 外文期刊>Infection and immunity >The rgg Gene of Streptococcus pyogenes NZ131 Positively Influences Extracellular SPE B Production
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The rgg Gene of Streptococcus pyogenes NZ131 Positively Influences Extracellular SPE B Production

机译：化脓性链球菌NZ131的rgg基因积极影响细胞外SPE B的产生

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Streptococcus pyogenes produces several extracellular proteins, including streptococcal erythrogenic toxin B (SPE B), also known as streptococcal pyrogenic exotoxin B and streptococcal proteinase. Several reports suggest that SPE B contributes to the virulence associated with S. pyogenes; however, little is known about its regulation. Nucleotide sequence data revealed the presence, upstream of the speB gene, of a gene, designatedrgg, that was predicted to encode a polypeptide similar to previously described positive regulatory factors. The putative Rgg polypeptide of S. pyogenes NZ131 consisted of 280 amino acids and had a predicted molecular weight of 33,246. To assess the potential role of Rgg in the production of SPE B, the rgggene was insertionally inactivated in S. pyogenes NZ131, which resulted in markedly decreased SPE B production, as determined both by immunoblotting and caseinolytic activity on agar plates. However, the production of other extracellular products, including streptolysin O, streptokinase, and DNase, was not affected. Complementation of the rgg mutant with an intactrgg gene copy in S. pyogenes NZ131 could restore SPE B production and confirmed that the rgg gene product is involved in the production of SPE B.
机译：化脓性链球菌会产生几种细胞外蛋白，包括链球菌红原性毒素B（SPE B），也称为链球菌热原性外毒素B和链球菌蛋白酶。一些报告表明，SPE B有助于与 S相关的毒力。化脓;但是，对其法规知之甚少。核苷酸序列数据揭示了在 speB 基因上游存在一个名为 rgg 的基因，该基因预计编码与前述阳性调节因子相似的多肽。推定的 S的Rgg多肽。化脓菌 NZ131由280个氨基酸组成，预测分子量为33,246。为了评估Rgg在SPE B产生中的潜在作用，在 S中插入 rgg 基因使其失活。化脓性菌 NZ131，通过琼脂平板上的免疫印迹和酪蛋白水解活性测定，可显着降低SPE B的产量。但是，其他细胞外产物，包括链球菌溶血素O，链激酶和DNase的生产均不受影响。 rgg 突变体与 S中完整的 rgg 基因拷贝互补。化脓性 NZ131可以恢复SPE B的产生，并证实 rgg 基因产物与SPE B的产生有关。

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