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首页> 外文期刊>Infection and immunity >Up-Regulation of Fas (CD95) and Induction of Apoptosis in Intestinal Epithelial Cells by Nematode-Derived Molecules
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Up-Regulation of Fas (CD95) and Induction of Apoptosis in Intestinal Epithelial Cells by Nematode-Derived Molecules

机译：线虫衍生分子上调Fas（CD95）并诱导肠道上皮细胞凋亡

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Infection by the intestinal nematode Nippostrongylus brasiliensis induces acceleration of apoptosis in the small intestinal villus epithelial cells in vivo. In the present study, we examined whether worm extract or excretory-secretory product induces apoptosis in the rat intestinal epithelial cell line IEC-6 in vitro. In the presence of worm extract or excretory-secretory product (≥6 μg/ml), IEC-6 cell growth was significantly suppressed, and there was a concomitant increase in the number of detached cells in culture dishes. Detached cells showed nuclear fragmentation, activation of caspase-3, and specific cleavage of poly(ADP-ribose) polymerase, suggesting that apoptosis was induced in these cells. Semiquantitative reverse transcription-PCR showed that expression of Fas (CD95) mRNA was up-regulated as early as 6 h after addition of excretory-secretory product, while Fas ligand expression and p53 expression were not up-regulated. Fluorescence-activated cell sorter analyses revealed a significant increase in Fas expression and a slight increase in FasL expression in IEC-6 cells cultured in the presence of excretory-secretory product, while control IEC-6 cells expressed neither Fas or FasL. These results indicated that N. brasiliensis worms produce and secrete biologically active molecules that trigger apoptosis in intestinal epithelial cells together with up-regulation of Fas expression, although the mechanism of induction of apoptosis remains to be elucidated.
机译：巴西肠线虫 Nippostrongylus brasiliensis 的感染可诱导体内小肠绒毛上皮细胞凋亡的加速。在本研究中，我们检查了蠕虫提取物或排泄分泌产物是否在体外诱导大鼠肠上皮细胞系IEC-6的凋亡。在蠕虫提取物或排泄分泌物（≥6μg/ ml）的存在下，IEC-6细胞的生长被显着抑制，并且培养皿中分离细胞的数量随之增加。分离的细胞显示出核分裂，caspase-3的活化以及聚（ADP-核糖）聚合酶的特异性裂解，表明在这些细胞中诱导了凋亡。半定量逆转录-PCR显示，Fas（CD95）mRNA的表达最早在添加排泄-分泌产物后6小时上调，而Fas配体表达和p53表达未上调。荧光激活细胞分选仪分析显示，在存在排泄分泌产物的情况下培养的IEC-6细胞中，Fas表达显着增加，而FasL表达则略有增加，而对照IEC-6细胞均未表达Fas或FasL。这些结果表明 N。巴西利亚蠕虫会产生并分泌生物活性分子，这些分子会触发肠道上皮细胞的凋亡以及Fas表达的上调，尽管尚需阐明诱导凋亡的机制。

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