Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages, in Standing versus Shaking Cultures, and under Low Oxygen Conditions

Anjan Purkayastha; Lee Ann McCue; Kathleen A. McDonough

首页> 外文期刊>Infection and immunity >Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages, in Standing versus Shaking Cultures, and under Low Oxygen Conditions

【24h】

Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages, in Standing versus Shaking Cultures, and under Low Oxygen Conditions

机译：在低氧条件下静置培养和摇动培养中，结核分枝杆菌推定的经典硝基还原酶基因的表达与巨噬细胞中acr基因的表达相互关联

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Tuberculosis remains a leading killer worldwide, and new approaches for its treatment and prevention are urgently needed. This effort will benefit greatly from a better understanding of gene regulation in Mycobacterium tuberculosis, particularly with respect to this pathogen's response to its host environment. We examined the behavior of two promoters from the divergently transcribed M. tuberculosis genes acr/hspX/Rv2031c (α-crystallin homolog) and Rv2032/acg (acr-coregulated gene) by using a promoter-GFP fusion assay in Mycobacterium bovis BCG. We found that Rv2032 is a novel macrophage-induced gene whose expression is coregulated with that of acr. Relative levels of intracellular induction for both promoters were significantly affected by shallow standing versus shaking bacterial culture conditions prior to macrophage infection, and both promoters were strongly induced under low oxygen conditions. Deletion analyses showed that DNA sequences within a 43-bp region were required for expression of these promoters under all conditions. Multiple sequence alignment and database searches performed with PROBE indicated that Rv2032 is one of eight M. tuberculosis genes of previously unknown function that belong to an unusual superfamily of classical nitroreductases, which may have a role for bacteria within the host environment. These findings show that mycobacterial culture conditions can greatly influence the results and interpretation of subsequent gene regulation experiments. We propose that these differences might be exploited for dissection of the regulatory factors that affect mycobacterial gene expression within the host.

机译：结核病仍然是世界范围内的主要杀手，因此迫切需要新的治疗和预防方法。更好地了解结核分枝杆菌中的基因调控，尤其是就该病原体对其宿主环境的反应而言，这项工作将大大受益。我们检查了来自不同转录的 M的两个启动子的行为。结核基因 acr / hsp X / Rv2031c（α-晶状体蛋白同源物）和Rv2032 / acg （ acr BCG中使用启动子-GFP融合检测的方法。我们发现Rv2032是一种新型的巨噬细胞诱导基因，其表达与 acr 的表达紧密相关。在巨噬细胞感染之前，浅站立和摇动细菌培养条件对两种启动子的细胞内诱导的相对水平有显着影响，并且两种启动子在低氧条件下被强烈诱导。缺失分析表明在所有条件下表达这些启动子都需要43bp区域内的DNA序列。用PROBE进行的多个序列比对和数据库搜索表明Rv2032是八个 M之一。以前未知功能的结核基因属于经典的硝基还原酶的超家族，可能对宿主环境中的细菌起作用。这些发现表明分枝杆菌培养条件可以极大地影响结果和后续基因调控实验的解释。我们建议这些差异可用于解剖影响宿主内分枝杆菌基因表达的调节因子。 展开▼

著录项

来源
《Infection and immunity》 |2002年第3期|共12页

作者
Anjan Purkayastha; Lee Ann McCue; Kathleen A. McDonough;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类医学免疫学;

关键词

相似文献

外文文献

中文文献

专利

1. Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages, in Standing versus Shaking Cultures, and under Low Oxygen Conditions [J] . Anjan Purkayastha, Lee Ann McCue, Kathleen A. McDonough Infection and immunity . 2002,第3期

机译：在低氧条件下静置培养和摇动培养中，结核分枝杆菌推定的经典硝基还原酶基因的表达与巨噬细胞中acr基因的表达相互关联

2. Expression of the Mycobacterium tuberculosis acr-Coregulated Genes from the DevR (DosR) Regulon Is Controlled by Multiple Levels of Regulation [J] . Hema M. Vasudeva-Rao, Kathleen A. McDonough Infection and immunity . 2008,第6期

机译：来自DevR（DosR）调节基因的结核分枝杆菌acr核心基因的表达受多个调控水平的控制

3. A Family of acr-Coregulated Mycobacterium tuberculosis Genes Shares a Common DNA Motif and Requires Rv3133c (dosR or devR) for Expression [J] . Matthew A. Florczyk, Lee Ann McCue, Anjan Purkayastha, Infection and immunity . 2003,第9期

机译：Acr核心化结核分枝杆菌基因家族共享一个共同的DNA图案，并且需要Rv3133c（dosR或devR）表达

4. The Effect of Low Oxygen With and Without Steady-state Hydrogen Peroxide on Cytokine Gene and Protein Expression of Monocyte-Derived Macrophages [C] . Henry Owegi, Hongtao Li, Mark Bouwens, International ISA biomedical sciences instrumentation symposium;Annual rocky mountain bioengineering symposium . 2011

机译：有和没有稳态过氧化氢的低氧对单核细胞衍生巨噬细胞细胞因子基因和蛋白质表达的影响

5. Bioinformatic and Sequence Analysis of Four Resuscitation Promoting Factor (Rpf) Gene Homologues in Mycobacterium avium subsp. paratuberculosis (Mpt), and Expression of the Putative Mpt rpfB in Escherichia coli. [D] . Hedlund, David D. 2010

机译：鸟分枝杆菌亚种中四个复苏促进因子（Rpf）基因同源物的生物信息学和序列分析。副结核病（Mpt）和假定的Mpt rpfB在大肠杆菌中的表达。

6. Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages in Standing versus Shaking Cultures and under Low Oxygen Conditions [O] . Anjan Purkayastha, Lee Ann McCue, Kathleen A. McDonough 2002

机译：在低氧条件下静置培养和摇动培养中结核分枝杆菌推定的经典硝基还原酶基因的表达与巨噬细胞中acr基因的表达相互关联

7. Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages, in Standing versus Shaking Cultures, and under Low Oxygen Conditions [O] . Purkayastha, Anjan, McCue, Lee Ann, McDonough, Kathleen A. 2002

机译：在低氧条件下静置培养和摇动培养中，结核分枝杆菌推定的经典硝基还原酶基因的表达与巨噬细胞中acr基因的表达相关的鉴定

1. 脆性组氨酸三聚体基因和含有WW结构域的氧化还原酶基因基因在喉癌中的表达及其相关性 [J] . 朱洪源 ,潘兆虎 ,吴国民 . 中华实验外科杂志 . 2018,第006期

2. 羰基还原酶基因与葡萄糖脱氢酶基因在大肠杆菌中的共表达及其在不对称还原产麻黄碱中的初步应用 [J] . 宇文伟刚 ,张梁 ,王正祥 . 工业微生物 . 2010,第003期

3. 低氧胁迫与常氧条件下斑马鱼鳃中热休克蛋白基因家族的表达差异比较 [J] . 狄治朝 ,周涛 ,许强华 . 大连海洋大学学报 . 2018,第006期

4. 低氧胁迫与常氧条件下斑马鱼鳃中热休克蛋白基因家族的表达差异比较 [J] . 狄治朝1 ,周涛1 ,许强华1234 . 大连海洋大学学报 . 2018,第006期

5. 切除修复交叉互补酶基因、核糖核苷酸还原酶M1、乳腺癌易感基因1在进展期肺癌组织中的表达及预测意义 [J] . 王晓艳 ,赵玉华 . 中国基层医药 . 2014,第004期

6. Klebsiella pneumoniae中醛还原酶基因的克隆与表达 [C] . 张乐 ,马成伟 ,戴建英 . 第五届全国化工年会 . 2008

7. Pandoraea sp.BD-33硝基苯硝基还原酶基因的克隆、表达及其酶性质研究 [A] . 胡华 . 2011

1. 在小麦根中特异表达的肉桂酰辅酶A还原酶、编码该酶的基因和含有该基因的表达载体 [P] . 中国专利： CN1264982C . 2006.07.19

2. 在小麦根中特异表达的肉桂酰辅酶A还原酶、编码该酶的基因和含有该基因的表达载体 [P] . 中国专利： CN1534095A . 2004-10-06

3. Methods for directing the expression of a target gene specific tissue in a plant, in the fruit of a plant, inside the seed of a plant, in a flower of a plant.The inside of a tuber of a plant, bud of a target gene in a plant, in a vascular system of a plant within a plant meristem inside the pollen of a plant.And inside an egg plant, seed, and methods to increase the biomass of a plant grown under one or more adverse environmental conditions.In order to increase the biomass of a plant growing under conditions of low nitrogen, to increase the production of seeds of a plant.To drive the expression of a target gene specific tissue within the root of a plant, and in a bud of a plant [P] . 外国专利： BR0107900A . 2002-11-05

机译：指导目标基因特异性组织在植物中，植物果实中，植物种子内，植物花中表达的方法。植物块茎内部，目标基因的芽在植物中，在植物花粉内的植物分生组织内，在植物的血管系统中，在茄子，种子内以及增加在一种或多种不利环境条件下生长的植物的生物量的方法中。增加在低氮条件下生长的植物的生物量，增加植物的种子产量。驱动目标基因特异性组织在植物根部和植物芽中的表达

相关主题

Identification of a Mycobacterium tuberculosis Putative Classical Nitroreductase Gene Whose Expression Is Coregulated with That of the acr Gene within Macrophages, in Standing versus Shaking Cultures, and under Low Oxygen Conditions

摘要

著录项

相似文献

相关主题

期刊订阅