Helicobacter pylori infection status following experimental inoculation of mice presently requires euthanasia. The purpose of this study was to develop a method for following the time course of H. pylori infection in live experimental animals. Twenty-six C57BL/6, Helicobacter-free female mice were inoculated with H. pylori Sydney strain 1, and 16 mice were sham inoculated. The mice were repeatedly tested during a period of about 1 year with an H. pylori species-specific primer-based PCR analysis of DNA extracted from fecal pellets of mice. The mice were euthanized at 6 months (n = 15) and 10 months (n = 15) to determine their infection status by histology, culture, and PCR of gastric specimens. H. pylori-inoculated mice were tested via the PCR method at 6 and 10 months prior to necropsy. Nine of 13 (69%) and 10 of 13 (77%) mice tested at 6 and 10 months, respectively, were positive. All sham-inoculated mice were negative. These two PCR results suggested a specificity of 100% with a sensitivity range between 69 and 77%. In contrast, sensitivity and specificity rose to 90 and 100% if groups of mice were tested once daily for 4 days. Seventy-seven to 85% of the experimental mice were also positive for H. pylori by culture. The histopathology demonstrated mild to severe gastritis. These findings demonstrate that the persistence or transience of H. pylori infection in live mice can be repeatedly evaluated over time. This method could allow the determination of the time course of infection and the efficacy of medications and/or vaccine without necropsy.
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机译：实验性接种小鼠后的幽门螺杆菌感染状态目前需要安乐死。这项研究的目的是开发一种追踪 H时程的方法。在活的实验动物中感染幽门螺杆菌。将26只C57BL / 6，无幽门螺杆菌的雌性小鼠接种 H。幽门螺杆菌悉尼毒株1，并假接种16只小鼠。在大约1年的时间内用 H对小鼠进行了反复测试。从小鼠粪便中提取DNA的基于幽门螺杆菌物种特异性引物的PCR分析。在第6个月（ n = 15）和第10个月（ n = 15）对小鼠实施安乐死，以通过组织学，培养和胃标本PCR来确定其感染状况。 H。尸检前6个月和10个月，通过PCR方法检测了接种幽门螺杆菌的小鼠。在6和10个月时分别测试了13只小鼠中的9只（69％）和13只小鼠中的10只（77％）是阳性。所有假接种的小鼠均为阴性。这两个PCR结果表明特异性为100％，灵敏度范围为69％至77％。相比之下，如果每天对小鼠进行一次连续4天的测试，其敏感性和特异性将分别提高到90％和100％。 77％至85％的实验小鼠的 H也呈阳性。幽门螺杆菌组织病理学表明轻度至重度胃炎。这些发现证明 H的持久性或瞬时性。随着时间的推移，可以反复评估活小鼠中的幽门螺杆菌感染。该方法可以确定感染的时间过程以及无需尸检的药物和/或疫苗的功效。
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