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首页> 外文期刊>Infection and immunity >Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7
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Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

机译：益生菌双歧杆菌可保护小鼠免受产志贺毒素的大肠杆菌O157：H7的致死性感染

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The anti-infectious activity of probiotic Bifidobacteria against Shiga toxin-producing Escherichia coli (STEC) O157:H7 was examined in a fatal mouse STEC infection model. Stable colonization of the murine intestines was achieved by the oral administration of Bifidobacterium breve strain Yakult (naturally resistant to streptomycin sulfate) as long as the mice were treated with streptomycin in their drinking water (5 mg/ml). The pathogenicity of STEC infection, characterized by marked body weight loss and subsequent death, observed in the infected controls was dramatically inhibited in the B. breve-colonized group. Moreover, Stx production by STEC cells in the intestine was almost completely inhibited in the B. breve-colonized group. A comparison of anti-STEC activity among several Bifidobacterium strains with natural resistance to streptomycin revealed that strains such as Bifidobacterium bifidum ATCC 15696 and Bifidobacterium catenulatum ATCC 27539T did not confer an anti-infectious activity, despite achieving high population levels similar to those of effective strains, such as B. breve strain Yakult and Bifidobacterium pseudocatenulatum DSM 20439. The effective strains produced a high concentration of acetic acid (56 mM) and lowered the pH of the intestine (to pH 6.75) compared to the infected control group (acetic acid concentration, 28 mM; pH, 7.15); these effects were thought to be related to the anti-infectious activity of these strains because the combination of a high concentration of acetic acid and a low pH was found to inhibit Stx production during STEC growth in vitro.
机译：在致命的小鼠STEC感染模型中，检查了益生菌双歧杆菌对产志贺毒素的大肠杆菌（Osc：O157：H7）的抗感染活性。只要口服饮用水中的链霉素处理小鼠，即可通过口服短双歧杆菌菌株Yakult（对硫酸链霉素具有天然抗性）来实现小鼠肠道的稳定定居。 。在感染的对照组中观察到的STEC感染的病原性以明显的体重减轻和随后的死亡为特征，在 B中被大大抑制。简短-定居组。而且， B中肠中STEC细胞产生的Stx几乎被完全抑制。简短-定居组。对几种对链霉素具有天然抗性的双歧杆菌菌株的抗STEC活性进行比较，结果显示，双歧双歧杆菌ATCC 15696和链双歧杆菌尽管达到了与有效菌株如 B相似的高种群水平，但27539 T 并未赋予抗感染活性。短株 Yakult和假双歧杆菌 DSM20439。与感染的相比，有效菌株产生高浓度的乙酸（56 mM）并降低了肠道的pH（至pH 6.75）。对照组（乙酸浓度，28 mM; pH，7.15）;这些作用被认为与这些菌株的抗感染活性有关，因为发现高浓度的乙酸和低的pH值的结合会抑制STEC体外生长过程中Stx的产生。

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