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首页> 外文期刊>Infection and immunity >Mechanisms of action of Pseudomonas aeruginosa pyocyanin on human ciliary beat in vitro.
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Mechanisms of action of Pseudomonas aeruginosa pyocyanin on human ciliary beat in vitro.

机译：铜绿假单胞菌绿脓素对人睫状跳动的体外作用机理。

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Pyocyanin is a blue redox active pigment produced by Pseudomonas aeruginosa. It is present at concentrations of up to 10(-4) M in sputa from patients with cystic fibrosis and bronchiectasis who are heavily colonized with this organism. Pyocyanin, at physiologically relevant concentrations, slows human nasal ciliary beat frequency (CBF) in vitro and leads to disruption of the epithelium. Pyocyanin-induced slowing of CBF after 2 h was associated with a significant fall in intracellular cyclic AMP (cAMP) (90%) and ATP (66%) and was reversible after the pyocyanin was removed by washing. These effects were not mediated through interaction with neutrophils. The pyocyanin-induced fall in CBF was not affected by EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid], pyrazinamide, 8-phenyltheophylline, indomethacin, or antioxidants, including catalase (500 U/ml), superoxide dismutase, and N-acetylcysteine. Ciliary slowing was, however, prevented (> 70%) by isobutylmethylxanthine and forskolin, both of which increase intracellular cAMP, and also by the cAMP analog, dibutyryl cAMP. There was also a concomitant protection against the fall in both cAMP and ATP. These agents also delayed the onset of epithelial disruption associated with pyocyanin treatment. In contrast, treatment with the iron chelator desferrioxamine prevented epithelial disruption, although it had no effect on pyocyanin-induced slowing of CBF. It appears that ciliary slowing can be dissociated from epithelial disruption and that the effects of pyocyanin on CBF are associated with a fall in both intracellular cAMP and ATP.
机译：绿脓素是一种由铜绿假单胞菌（Pseudomonas aeruginosa）生产的蓝色氧化还原活性颜料。患有该病原体的囊性纤维化和支气管扩张患者的唾液中浓度高达10（-4）M。生理相关浓度的花青素在体外会减慢人鼻睫状跳动频率（CBF），并导致上皮细胞破坏。 2小时后，花青素诱导的CBF减慢与细胞内环状AMP（cAMP）（90％）和ATP（66％）的显着下降有关，并且在通过洗涤去除了花青素后是可逆的。这些作用不是通过与中性粒细胞的相互作用介导的。绿脓杆菌诱导的CBF下降不受EGTA [乙二醇-双（β-氨基乙基醚）-N，N，N'，N'-四乙酸]，吡嗪酰胺，8-苯基茶碱，消炎痛或抗氧化剂的影响，包括过氧化氢酶（500 U / ml），超氧化物歧化酶和N-乙酰半胱氨酸。但是，异丁基甲基黄嘌呤和福司可林可防止纤毛变慢（> 70％），这两者均会增加细胞内cAMP，也可通过cAMP类似物二丁酰cAMP来防止。同时还提供了针对cAMP和ATP下降的保护作用。这些试剂还延迟了与黄绿素治疗相关的上皮破坏的发作。相反，用铁螯合剂去铁草胺治疗可预防上皮细胞破坏，尽管它对洋蓝蛋白诱导的CBF减慢没有影响。看来睫状体减慢可以从上皮破坏中解离出来，并且花青素对CBF的作用与细胞内cAMP和ATP的下降有关。

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