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首页> 外文期刊>Infection and immunity >Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F.
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Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F.

机译：19F型肺炎链球菌荚膜多糖生物合成必不可少的基因的核苷酸序列分析。

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Previous studies have shown that the capsular polysaccharide synthesis (cps) locus of the type 19F Streptococcus pneumoniae strain SSZ was closely linked to a copy of the insertion sequence IS1202 (J.K. Morona, A. Guidolin, R. Morona, D. Hansman, and J.C. Paton, J. Bacteriol. 176:4437-4443, 1994). In the present study, we used plasmid insertion and rescue and inverse PCR to clone 6,322 bp of flanking DNA upstream of IS1202. Sequence analysis indicated that this region contains six complete open reading frames (ORFs) and one partial ORF that are arranged as a single transcriptional unit. Chromosomal disruption of any of these ORFs in a smooth-type 19F strain leads to a rough (unencapsulated) phenotype, indicating that this operon is essential for capsule production. The ORFs have therefore been designated cps19fA to cps19fG, where cps19fA is the first gene of the type 19F cps locus. Furthermore, many of the gene products from this incomplete operon exhibit strong similarities to proteins known to be involved in the production of capsular polysaccharide, exopolysaccharide, teichoic acid, enterobacterial common antigen, and lipopolysaccharide from numerous other bacterial species. This has allowed us to propose functions for many of the type 19F cps gene products. Southern hybridization studies reveal that cps19fA and cps19fB are conserved among all 12 pneumococcal serotypes tested, whereas genes downstream of cps19fB are conserved among some, but not all, of the serotypes tested.
机译：先前的研究表明，19F型肺炎链球菌肺炎链球菌菌株SSZ的荚膜多糖合成（cps）位点与插入序列IS1202的副本紧密相连（JK Morona，A。Guidolin，R。Morona，D。Hansman和JC Paton，J.Bacteriol.176：4437-4443，1994）。在本研究中，我们使用质粒插入，拯救和反向PCR克隆了IS1202上游侧翼DNA的6,322 bp。序列分析表明，该区域包含六个完整的开放阅读框（ORF）和一个部分ORF，它们被安排为一个转录单位。在光滑型19F菌株中，任何这些ORF的染色体破坏都会导致粗糙的（未封装）表型，表明该操纵子对于胶囊生产至关重要。因此，ORF被命名为cps19fA到cps19fG，其中cps19fA是19F cps类型的第一个基因。此外，来自这种不完整操纵子的许多基因产物与已知参与荚膜多糖，胞外多糖，硫磷酸，肠细菌共同抗原和来自许多其他细菌物种的脂多糖生产相关的蛋白质表现出强烈的相似性。这使我们能够为许多19F cps型基因产物提出功能。 Southern杂交研究表明，cps19fA和cps19fB在所测试的所有12种肺炎球菌血清型中均是保守的，而cps19fB下游的基因在某些但并非全部血清型中是保守的。

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