...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Pasteurella haemolytica lipopolysaccharide-associated protein induces pulmonary inflammation after bronchoscopic deposition in calves and sheep.
【24h】

Pasteurella haemolytica lipopolysaccharide-associated protein induces pulmonary inflammation after bronchoscopic deposition in calves and sheep.

机译：溶血巴斯德氏菌脂多糖相关蛋白在小牛和绵羊的支气管镜下沉积后诱发肺部炎症。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The lipopolysaccharide (LPS)-associated protein (LAP) was extracted from Pasteurella haemolytica serotype A1 strains L101 (bovine origin) and 82-25 (ovine origin). Extracts contained 0.017% total LPS and appeared as only two bands at 14 and 16.6 kDa after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. To determine the extent of pulmonary inflammation induced by LAP and its possible role in the pathogenesis of pneumonic pasteurellosis, LAP (500 micrograms in pyrogen-free saline [PFS]) was deposited by fiber-optic bronchoscopy into the dorsum of the caudal portion of the cranial lobe of the right lung of calves (strain L101 LAP) and sheep (strain 82-25 LAP). LPS (500 micrograms in PFS), 3-h P. haemolytica cultures (1.6 x 10(8) to 1.9 x 10(8) CFU in PFS), and PFS alone were deposited similarly as controls. At necropsy, 24 h after deposition, gross and histologic pulmonary lesions of calves and sheep given LAP, LPS, and P. haemolytica were similar and consisted of various degrees of acute bronchopneumonia (relative severities of lesions induced: LAP < LPS < live organisms). By subjective histologic interpretation and semiquantitative morphometry, animals given LAP had the highest percentage of macrophages per alveolar lumen and the lowest percentage of neutrophils. The lesions from animals given LPS were more severe than those given LAP, but the morphometric cell counts were similar. In contrast, animals inoculated with P. haemolytica had lesions typical of this agent, consisting of many neutrophils, proteinaceous exudate, and a few macrophages. Morphometrically, these lesions had the highest numbers of neutrophils and the lowest numbers of macrophages. These studies show that LAP can induce an inflammatory response in the alveolar lumens and may play a role in the pathogenesis of pneumonic pasteurellosis.
机译：脂多糖（LPS）相关蛋白（LAP）从溶血巴斯德氏菌血清型A1菌株L101（牛源）和82-25（羊源）中提取。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，提取物含有0.017％的总LPS，在14和16.6 kDa处仅显示为两个谱带。为了确定由LAP引起的肺部炎症的程度及其在肺巴氏杆菌病发病机理中的可能作用，通过纤维支气管镜将LAP（500微克无热原盐水[PFS]）沉积到肺的尾部背部小牛（L101 LAP株）和绵羊（82-25 LAP株）右肺的颅叶。 LPS（PFS中为500微克），3-h溶血性毕赤酵母培养物（PFS中为1.6 x 10（8）至1.9 x 10（8）CFU）和单独的PFS与对照相似地沉积。尸检时，在沉积后24小时，给予LAP，LPS和溶血性疟原虫的小牛和绵羊的肉眼和组织学肺部病变相似，且由不同程度的急性支气管肺炎组成（病变的相对严重程度：LAP

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号