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首页> 外文期刊>Infection and immunity >Campylobacter jejuni motility and invasion of Caco-2 cells.
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Campylobacter jejuni motility and invasion of Caco-2 cells.

机译：空肠弯曲杆菌的运动性和侵袭Caco-2细胞。

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摘要

We investigated the influence of motility on Campylobacter jejuni binding and invasion of Caco-2 cells. C. jejuni was motile in soft agar at basic (pH 8.5) and neutral pH values representative of the intestinal environment. However, C. jejuni was immobilized at pH 5.0. The inability of C. jejuni to swarm on soft agar at pH 5.0 was not related to flagellar depolymerization or loss of viability. In tissue culture medium, C. jejuni displayed typical periods of straight swimming punctuated by tumbling behavior. This behavior was altered when the viscosity of the medium was adjusted to mimic the viscosity of intestinal mucus. C. jejuni showed longer periods of straight swimming with significantly increased velocity followed by pauses instead of tumbles. The binding and invasion of C. jejuni in Caco-2 cells also increased significantly in high-viscosity growth medium. We speculate that the swimming behavior of C. jejuni in a viscous environment may be an important factor in the interaction of these organisms with host epithelial cells. The pH, which affects C. jejuni motility, may also influence the tropism of these organisms.
机译：我们调查了运动对空肠弯曲杆菌结合和侵袭Caco-2细胞的影响。空肠弯曲杆菌在碱性（pH 8.5）和中性pH值（代表肠道环境）中在软琼脂中运动。然而，空肠弯曲杆菌被固定在pH 5.0。空肠弯曲杆菌无法在pH 5.0的软琼脂上成群生长与鞭毛解聚或活力丧失无关。在组织培养基中，空肠弯曲杆菌表现出典型的直立游泳时期，其间有翻滚行为。当调节培养基的粘度以模拟肠粘液的粘度时，这种行为会改变。空肠弯曲杆菌显示出较长的直泳时间，其速度明显增加，随后停顿而不是翻滚。在高粘度生长培养基中，空肠弯曲杆菌在Caco-2细胞中的结合和侵袭也显着增加。我们推测空肠弯曲杆菌在粘性环境中的游泳行为可能是这些生物与宿主上皮细胞相互作用的重要因素。影响空肠弯曲杆菌运动性的pH值也可能影响这些生物的向性。

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