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首页> 外文期刊>Infection and immunity >Binding of mannose-binding protein to Klebsiella O3 lipopolysaccharide possessing the mannose homopolysaccharide as the O-specific polysaccharide and its relation to complement activation.
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Binding of mannose-binding protein to Klebsiella O3 lipopolysaccharide possessing the mannose homopolysaccharide as the O-specific polysaccharide and its relation to complement activation.

机译：甘露糖结合蛋白与具有甘露糖同多糖作为O-特异性多糖的克雷伯氏菌O3脂多糖的结合及其与补体激活的关系。

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摘要

Lipopolysaccharide from Klebsiella pneumoniae O3, which possesses the mannose homopolysaccharide as the O-specific polysaccharide, exhibits an extraordinarily high ability to activate the human complement system. We isolated the mannose-binding protein with a Klebsiella O3 lipopolysaccharide affinity column. The protein isolated had a molecular mass of much higher than 200 kDa, and it consisted of subunits with an apparent molecular mass of 32 kDa. The NH2-terminal sequence of the 32-kDa subunits was completely consistent with a part of the amino acid sequence of human serum mannose-binding protein. In immunoblotting, an anti-mannose-binding protein monoclonal antibody was definitely reactive with the isolated protein with the higher molecular mass. The protein isolated was bound exclusively to lipopolysaccharides possessing the mannose homopolysaccharide, not to lipopolysaccharide possessing the heteropolysaccharides. Klebsiella O3 lipopolysaccharide did not exhibit a high anticomplement activity in the serum from which the mannose-binding protein was depleted. It was concluded that the serum factor that bound to Klebsiella O3 lipopolysaccharide may be mannose-binding protein and that it may play a crucial role in the strong complement activation by Klebsiella O3 lipopolysaccharide.
机译：来自肺炎克雷伯菌O3的脂多糖具有甘露糖均多糖作为O特异性多糖，具有极高的激活人补体系统的能力。我们用克雷伯菌O3脂多糖亲和柱分离了甘露糖结合蛋白。分离出的蛋白质的分子量远高于200 kDa，并且由表观分子量为32 kDa的亚基组成。 32-kDa亚基的NH 2-末端序列与人血清甘露糖结合蛋白的氨基酸序列的一部分完全一致。在免疫印迹中，抗甘露糖结合蛋白单克隆抗体肯定会与分子量较高的分离蛋白发生反应。分离出的蛋白质仅与具有甘露糖同多糖的脂多糖结合，而不与具有杂多糖的脂多糖结合。克雷伯菌O3脂多糖在耗尽了甘露糖结合蛋白的血清中没有表现出高的抗补体活性。得出的结论是，与克雷伯菌O3脂多糖结合的血清因子可能是甘露糖结合蛋白，它可能在克雷伯菌O3脂多糖的强补体激活中起关键作用。

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