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首页> 外文期刊>Infection and immunity >Induction of adherence and degranulation of polymorphonuclear leukocytes: a new expression of the invasive phenotype of Shigella flexneri.
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Induction of adherence and degranulation of polymorphonuclear leukocytes: a new expression of the invasive phenotype of Shigella flexneri.

机译：多形核白细胞粘附和脱粒的诱导：志贺氏志贺氏菌侵袭性表型的新表达。

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摘要

In the present study, the ability of Shigella flexneri to activate polymorphonuclear neutrophils (PMN) was examined. The invasive serotype 5 strain M90T induced strong PMN adherence, which was dependent on both the multiplicity of infection and the duration of incubation. When tested under the same experimental conditions, the noninvasive strain BS176 (cured of the 220-kb virulence plasmid) was less efficient. Indeed, incubation of PMN for 2 h with either M90T or BS176 (multiplicity of infection, 100) induced 51.8% +/- 10.5% and 15.2% +/- 4.2% adherence, respectively (n = 3; P < 0.05). Stronger PMN activation by M90T was confirmed by evaluating PMN degranulation induced by the two strains. Whereas M90T triggered significant PMN secretion, BS176 did not. M90T strains with mutations in ipa genes were then analyzed. When PMN were incubated with these mutants, their activation was of the same intensity as that obtained with BS176. These data provide the first evidence for PMN activation induced by S. flexneri, a process which appears to be mediated by Ipa invasins.
机译：在本研究中，检查了弗氏志贺氏菌激活多形核中性粒细胞（PMN）的能力。侵袭性血清型5株M90T诱导强烈的PMN粘附，这取决于感染的多样性和孵育时间。在相同的实验条件下进行测试时，非侵入性菌株BS176（220 kb毒性质粒的固化）效率较低。确实，PMN与M90T或BS176孵育2小时（感染复数为100）分别引起51.8％+/- 10.5％和15.2％+/- 4.2％的依从性（n = 3; P <0.05）。通过评估由两种菌株诱导的PMN脱粒，证实了M90T对PMN的激活更强。 M90T触发了PMN的大量分泌，而BS176没有。然后分析了ipa基因突变的M90T菌株。当将PMN与这些突变体孵育时，它们的激活强度与BS176相同。这些数据提供了由弗氏链球菌诱导的PMN活化的第一个证据，该过程似乎是由Ipa入侵素介导的。

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