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首页> 外文期刊>Infection and immunity >Molecular and Functional Characterization ofSalmonella enterica Serovar Typhimurium poxAGene: Effect on Attenuation of Virulence and Protection
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Molecular and Functional Characterization ofSalmonella enterica Serovar Typhimurium poxAGene: Effect on Attenuation of Virulence and Protection

机译：肠炎沙门氏菌鼠伤寒沙门氏菌poxA基因的分子和功能表征：对毒力的减弱和保护作用。

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Salmonella enterica poxA mutants exhibit a pleiotropic phenotype, including reduced pyruvate oxidase activity; reduced growth rate; and hypersensitivity to the herbicide sulfometuron methyl, α-ketobutyrate, and amino acid analogs. These mutants also failed to grow in the presence of the host antimicrobial peptide, protamine. In this study, PoxA? mutants of S. entericaserovar Typhimurium (S. typhimurium) were found to be 10,000-fold attenuated in orally inoculated BALB/c mice and 1,000-fold attenuated in intraperitoneally inoculated BALB/c mice, compared to wild-type S. typhimurium UK-1. In addition,poxA mutants were found to be capable of colonizing the spleen, mesenteric lymph nodes, and Peyer’s patches; to induce strong humoral immune responses; and to protect mice against a lethal wild-type Salmonella challenge. A 2-kb DNA fragment was isolated from wild-type S. typhimurium UK-1 based on its ability to complement an isogenic poxA mutant. The nucleotide sequence of this DNA fragment revealed an open reading frame of 325 amino acids capable of encoding a polypeptide of 36.8 kDa that was confirmed in the bacteriophage T7 expression system. Comparison of the translated sequence to the available databases indicated high homology to a family of lysyl-tRNA synthetases. Our results indicate that a mutation of poxA has an attenuating effect onSalmonella virulence. Further, poxA mutants are immunogenic and could be useful in designing live vaccines with a variety of bacterial species. To our knowledge, this is the first report on the effect of poxA mutation on bacterial virulence.
机译：肠炎沙门氏菌poxA 突变体表现出多效性表型，包括丙酮酸氧化酶活性降低;增长率降低;对除草剂磺胺磺隆甲基，α-酮丁酸和氨基酸类似物过敏。这些突变体在宿主抗微生物肽鱼精蛋白的存在下也无法生长。在这项研究中， S的PoxA ？突变体。与野生型相比，口服接种的BALB / c小鼠肠炎血清鼠伤寒（鼠伤寒沙门氏菌）衰减10,000倍，腹膜内接种的BALB / c鼠肠炎血清鼠伤寒类型 S。鼠伤寒 UK-1。此外，还发现了 poxA 突变体能够在脾脏，肠系膜淋巴结和派伊尔氏斑中定植。诱导强烈的体液免疫反应;并保护小鼠免受野生型沙门氏菌的致命攻击。从野生型 S中分离出2-kb的DNA片段。鼠伤寒 UK-1具有互补同基因 poxA 突变体的能力。该DNA片段的核苷酸序列揭示了325个氨基酸的开放阅读框，其能够编码36.8kDa的多肽，这在噬菌体T7表达系统中得到了证实。将翻译的序列与可获得的数据库进行比较表明与赖氨酰-tRNA合成酶家族高度同源。我们的结果表明， poxA 突变对沙门氏菌毒力具有减毒作用。此外， poxA 突变体具有免疫原性，可用于设计具有多种细菌种类的活疫苗。据我们所知，这是关于 poxA 突变对细菌毒力影响的第一份报告。

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