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首页> 外文期刊>Infection and immunity >Expression of human choriogonadotropin-like material in coagulase-negative Staphylococcus species.
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Expression of human choriogonadotropin-like material in coagulase-negative Staphylococcus species.

机译：人绒毛膜促性腺激素样物质在凝固酶阴性葡萄球菌中的表达。

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We identified 101 coagulase-negative Staphylococcus strains obtained from different laboratories, the American Type Culture Collection, and our collection, isolated from 23 patients with overt cancer and 34 normal individuals through Kloos and Schleifer conventional methods and the Staph-Ident staphylococcal system (Analytab Products, Plainview, N.Y.). In 40 strains, identity was further verified by DNA-DNA hybridization techniques. Identification revealed 39 S. epidermidis, 22 S. hominis, 8 S. haemolyticus, 9 S. capitis, 5 S. warneri, 5 S. cohnii, 8 S. saprophyticus, and 5 S. xylosus strains, all resident species found in humans. All bacteria were tested for the expression of human choriogonadotropin (hCG)-like material by the indirect fluorescein and peroxidase immunocytochemical labeling techniques by using specific antisera to the whole hormone, to its alpha and beta subunits, to the hCG beta COOH-terminal peptide, and to a monoclonal antibody to the hCG beta. The results demonstrated that the isolates from cancer patients were not unique bacteria, as has been postulated by others; the expression of immunoreactive hCG-like material is a strain, not a species, characteristic; not every bacterial strain isolated from a cancer patient is able to express the material; hCG-producing bacteria do not necessarily indicate the presence of active disease; 20% of the strains that we studied revealed a clonal variation of the expression of hCG-like material or its subunits or both as well as a variable expression of a single hCG epitope, an observation similar to that described for malignant cells; and a specific antiserum to the whole hormone with a high affinity and high sensitivity for immunocytochemistry can be a reliable reagent for screening purposes.
机译：我们鉴定了101种从不同实验室，美国典型培养物保藏中心和我们实验室收集的凝固酶阴性葡萄球菌菌株，这些菌株通过Kloos和Schleifer常规方法以及金黄色葡萄球菌身份葡萄球菌系统（Analytab Products）从23名明显的癌症患者和34名正常个体中分离出来（纽约州普莱恩维尤）。在40个菌株中，通过DNA-DNA杂交技术进一步验证了同一性。鉴定显示，表皮葡萄球菌，人沙门氏菌22个，溶血链球菌8个，溶血链球菌9个，华氏链球菌5个，cohnii菌5个，腐生链球菌8个，腐生葡萄球菌5个。 。使用间接抗荧光素和过氧化物酶免疫细胞化学标记技术，通过使用针对整个激素，其α和β亚基，hCGβCOOH末端肽的特异性抗血清，通过间接荧光素和过氧化物酶免疫细胞化学标记技术对所有细菌的人绒毛膜促性腺激素（hCG）样材料的表达进行了测试。以及针对hCG beta的单克隆抗体。结果表明，从癌症患者中分离出的细菌并不是独特的细菌，正如其他人所推测的那样。免疫反应性hCG样物质的表达是一种菌株，而不是物种的特征。并非从癌症患者中分离出的每个细菌菌株都能够表达该物质;产生hCG的细菌不一定表明存在活动性疾病;我们研究的菌株中有20％揭示了hCG样材料或其亚基或两者的表达的克隆变异，以及单个hCG表位的可变表达，这一观察与恶性细胞相似。对免疫细胞化学具有高亲和力和高敏感性的针对全激素的特异性抗血清可以作为筛选目的的可靠试剂。

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