A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4.

M Norgren; M G Caparon; J R Scott

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A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4.

机译：一种使用共轭转座子Tn916的等位基因替换方法：化脓链球菌JRS4中的emm6.1等位基因缺失。

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The emm6.1 allele of Streptococcus pyogenes JRS4 was deleted by using the conjugative transposon Tn916. The aphA-3 gene, conferring resistance to kanamycin, was cloned between the sequences flanking the structural gene for the type 6 M protein (emm6.1) and inserted into the BstXI site of Tn916 to generate the chimeric transposon Tn916-5K3. Because the BstXI site lies in a nonessential region of Tn916, the chimeric transposon could transfer by conjugation from Bacillus subtilis into JRS4. In some of the transconjugants, Tn916-5K3 replaced the emm6.1 locus of JRS4 by homologous recombination between the cloned emm6.1-flanking regions and the resident chromosome. One recombinant studied in detail, JRS75, was kanamycin resistant and tetracycline sensitive and lacked immunologically detectable M6 protein. Furthermore, by Southern blot analysis, the DNA region encompassing the emm6.1 structural gene was found to have been replaced by aphA-3.

机译：化脓性链球菌JRS4的emm6.1等位基因通过使用转座子Tn916缺失。将赋予对卡那霉素抗性的aphA-3基因克隆到6 M型蛋白质结构基因（emm6.1）侧翼的序列之间，并插入Tn916的BstXI位点，以产生嵌合转座子Tn916-5K3。由于BstXI位点位于Tn916的非必需区域，因此嵌合转座子可以通过与枯草芽孢杆菌的结合转移到JRS4中。在某些转导结合体中，Tn916-5K3通过克隆的emm6.1侧翼区域与驻留染色体之间的同源重组取代了JRS4的emm6.1基因座。一种经过详细研究的重组体JRS75具有卡那霉素抗性和四环素敏感性，并且缺乏免疫学上可检测的M6蛋白。此外，通过Southern印迹分析，发现包含emm6.1结构基因的DNA区域已被aphA-3取代。

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《Infection and immunity》 |1989年第12期|共5页
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M Norgren; M G Caparon; J R Scott;
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1. A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4. [J] . M Norgren, M G Caparon, J R Scott Infection and immunity . 1989,第12期

机译：一种使用共轭转座子Tn916的等位基因替换方法：化脓链球菌JRS4中的emm6.1等位基因缺失。
2. Macrolide-resistant Streptococcus pyogenes from Chinese pediatric patients in association with Tn916 transposons family over a 16-year period. [J] . Feng L, Lin H, Ma Y, Diagnostic microbiology and infectious disease . 2010,第4期

机译：来自中国儿科患者的化脓性大环内酯化脓性链球菌与Tn916转座子家族在16年的时间里。
3. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. [J] . A E Franke, D B Clewell Journal of bacteriology . 1981,第1期

机译：粪链球菌中染色体携带的抗性转座子（Tn916）的证据，可在不存在结合质粒的情况下“结合”转移。
4. Erythromycin resistance associated to mefA gene and emm75 allele in Streptococcus pyogenes from uncomplicated pharyngitis in Mexico [C] . L.M. Perea-Mejia, C. Rivas, A.E. Inzunza-Montiel, Lancefield International Symposium on Streptococci and Streptococcal Diseases . 2006

机译：在墨西哥简单的咽炎中与MeFA基因和EMM75等位基因相关的红霉素抗性
5. A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4. [O] . M Norgren, M G Caparon, J R Scott 1989

机译：一种使用结合转座子Tn916的等位基因替换方法：化脓性链球菌JRS4中emm6.1等位基因的缺失。
6. A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4 [O] . M Norgren, M G Caparon, J R Scott 1989

机译：一种使用缀合性转座TN916的等位基因替代方法：缺失EMM6.1等位基因在链球菌化合物中的JRS4

A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4.

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