• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Expression of Tac antigen component of bovine interleukin-2 receptor in different leukocyte populations infected with Theileria parva or Theileria annulata.
【24h】

Expression of Tac antigen component of bovine interleukin-2 receptor in different leukocyte populations infected with Theileria parva or Theileria annulata.

机译：牛白细胞介素2受体Tac抗原成分在感染了Theileria parva或Theileria annulata的不同白细胞群体中的表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Tac antigen component of the bovine interleukin-2 receptor was expressed as a Cro-beta-galactosidase fusion protein in Escherichia coli and used to raise antibodies in rabbits. These antibodies were used for flow cytofluorimetric analysis to investigate the expression of Tac antigen in a variety of Theileria parva-infected cell lines and also in three Theileria annulata-infected cell lines. Cells expressing Tac antigen on their surface were found in all T. parva-infected cell lines tested whether these were of T- or B-cell origin. T cells expressing Tac antigen could be CD4- CD8-, CD4+ CD8-, CD4- CD8+, or CD4+ CD8+. Tac antigen expression was observed both in cultures which had been maintained in the laboratory for several years and in transformed cell lines which had recently been established by infection of lymphocytes in vitro with T. parva. Northern (RNA) blot analysis demonstrated Tac antigen transcripts in RNA isolated from all T. parva-infected cell lines. Three T. annulata-infected cell lines which were not of T-cell origin were also tested. Two of them expressed Tac antigen on their surface. Abundant Tac antigen mRNA was detected in these T. annulata-infected cell lines, but only trace amounts were demonstrated in the third cell line, which contained very few Tac antigen-expressing cells. In all cell lines tested, whether cloned or uncloned, a proportion of the cells did not express detectable levels of Tac antigen on their surface. This was also the case for a number of other leukocyte surface markers. In addition, we showed that the interleukin-2 receptors were biologically functional, because addition of recombinant interleukin-2 to cultures stimulated cell proliferation. Recombinant interleukin-2 treatment also resulted in increased amounts of steady-state Tac antigen mRNA. The relevance of interleukin-2 receptor expression on Theileria-infected cells is discussed.
机译：牛白介素2受体的Tac抗原成分在大肠杆菌中表达为Cro-β-半乳糖苷酶融合蛋白，并用于在兔中产生抗体。这些抗体用于流式细胞荧光分析，以研究Tac抗原在各种被Theileria parva感染的细胞系中以及在三个被Theileria annulata感染的细胞系中的表达。在所有经T. parva感染的细胞系中检测到在其表面表达Tac抗原的细胞，这些细胞系是否来自T细胞或B细胞。表达Tac抗原的T细胞可以是CD4- CD8-，CD4 + CD8-，CD4- CD8 +或CD4 + CD8 +。 Tac抗原的表达在实验室中已经维持了数年的培养物中，以及在最近被细小T. parva感染淋巴细胞而建立的转化细胞系中均被观察到。 Northern（RNA）印迹分析表明，Tac抗原转录本分离自所有感染了T. parva的细胞株。还测试了三种非T细胞来源的无环线虫感染的细胞系。其中两个在其表面表达Tac抗原。在这些感染了环线虫的细胞系中检测到了丰富的Tac抗原mRNA，但在含有很少Tac抗原表达细胞的第三种细胞系中仅显示了痕量。在所有测试的细胞系中，无论是克隆的还是未克隆的，一部分细胞在其表面均未表达可检测水平的Tac抗原。许多其他白细胞表面标志物也是如此。此外，我们表明白介素2受体具有生物学功能，因为向培养物中添加重组白介素2可以刺激细胞增殖。重组白介素2处理也导致稳态Tac抗原mRNA的数量增加。讨论了白细胞介素2受体表达在泰勒虫感染的细胞上的相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号