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Heat shock response of spirochetes.

机译:螺旋体的热冲击响应。

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We examined the heat shock response of the pathogenic spirochetes Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans and certain saprophytic spirochetes. Cellular proteins synthesized after shifts to higher temperatures were [35S]methionine labeled and analyzed by gel electrophoresis and fluorography. Only T. pallidum failed to exhibit an obvious heat shock response. GroEL and DnaK homologs were identified in the various species, although these proteins were not thermoinducible in T. pallidum or Treponema denticola. DNA hybridization studies indicate that spirochetal groEL and dnaK genes are highly conserved.
机译:我们检查了致病性螺旋体,梅毒螺旋体,伯氏疏螺旋体和问号钩端螺旋体以及某些腐生螺旋体的热休克反应。转变为更高温度后合成的细胞蛋白经过[35S]蛋氨酸标记,并通过凝胶电泳和荧光照相分析。只有苍白锥failed未能表现出明显的热激反应。在这些物种中鉴定出了GroEL和DnaK同源物,尽管这些蛋白在梅毒螺旋体或密螺旋体中不是可热诱导的。 DNA杂交研究表明,螺旋形groEL和dnaK基因是高度保守的。

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