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首页> 外文期刊>Infection and immunity >Purification and characterization of Streptococcus adjacens (nutritionally variant Streptococcus serotype II) group antigen.
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Purification and characterization of Streptococcus adjacens (nutritionally variant Streptococcus serotype II) group antigen.

机译：相邻链球菌（营养变异型链球菌血清型II）组抗原的纯化和鉴定。

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Nutritionally variant streptococci (NVS) possess amphiphiles which are serologically distinct from lipoteichoic acid and which serve as group-specific antigens for NVS. The objective of this study was to purify and characterize the NVS serotype II (Streptococcus adjacens) amphiphile. Amphiphile was isolated from stationary-phase culture supernatants of NVS strain 81 (NVS serotype II). Phenol-water extracts of culture supernatants were subjected to hydrophobic interaction chromatography and gel filtration chromatography. A homogeneous preparation of amphiphile (22 mg; 8.5 x 10(6) hemagglutination units) was recovered, and its approximate molecular size (23,000 to 24,000 Da) and chemical composition were determined. Purified S. adjacens amphiphile contained phosphorus, ribitol, galactose, galactosamine, alanine, and fatty acids in molar ratios of 1.00:0.88:1.39:1.10:0.08:0.24. Since ribitol, galactose, and galactosamine were the primary carbohydrate components, the amphiphile may exist as a polyribitol phosphate with galactose and galactosamine substituents. Preliminary structural analysis demonstrated the presence of phosphodiester bonds within the amphiphile structure. Finally, the amphiphile serves as the S. adjacens group antigen.
机译：营养变异链球菌（NVS）具有两亲性，两性在血清学上不同于脂磷壁酸，并且是NVS的组特异性抗原。这项研究的目的是纯化和鉴定NVS血清型II（相邻链球菌）两亲性。从NVS菌株81（NVS血清型II）的固定相培养上清液中分离出两亲物。将培养上清液的苯酚-水提取物进行疏水相互作用色谱和凝胶过滤色谱。回收均质的两亲物（22 mg; 8.5 x 10（6）血凝单位），并确定其近似分子大小（23,000至24,000 Da）和化学组成。纯化的S.adjacens两亲物含有磷，核糖醇，半乳糖，半乳糖胺，丙氨酸和脂肪酸的摩尔比为1.00：0.88：1.39：1.10：0.08：0.24。由于核糖醇，半乳糖和半乳糖胺是主要的碳水化合物成分，因此两亲物可以以具有半乳糖和半乳糖胺取代基的聚核糖醇磷酸酯的形式存在。初步的结构分析表明，两亲结构中存在磷酸二酯键。最后，两亲物作为邻近链球菌组抗原。

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